Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa: Chromosomal mapping and expression

Arnould Savouré, A. Fehér, P. Kaló, György Petrovics, Gyula Csanádi, Judit Szecsi, G. Kiss, Spencer Brown, Adam Kondorosi, É. Kondorosi

Research output: Article

34 Citations (Scopus)

Abstract

Cyclins in association with the protein kinase p34cdc2and related cyclin-dependent protein kinases (cdks) are key regulatory elements in controlling the cell division cycle. Here, we describe the identification and characterization of a full-length cDNA clone of alfalfa mitotic cyclin, termed CycIIIMs. Computer analysis of known plant cyclin gene sequences revealed that this cyclin belongs to the same structural group as the other known partial alfalfa cyclin sequences. Genetic segregation analysis based on DNA-DNA hybridization data showed that the CycIIIMs gene(s) locates in a single chromosomal region on linkage group 5 of the alfalfa genetic map between RFLP markers UO89A and CG13. The assignment of this cyclin to the mitotic cyclin class was based on its cDNA-derived sequence and its differential expression during G2/M cell cycle phase transition of a partially synchronized alfalfa cell culture. Sequence analysis indicated common motifs with both the A- and B-types of mitotic cyclins similarly to the newly described B3-type of animal cyclins.

Original languageEnglish
Pages (from-to)1059-1070
Number of pages12
JournalPlant Molecular Biology
Volume27
Issue number6
DOIs
Publication statusPublished - márc. 1995

Fingerprint

Cyclins
Medicago sativa
cyclins
chromosome mapping
Complementary DNA
Clone Cells
clones
alfalfa
protein kinases
Cell Cycle
Genes
Cyclin B
Cells
Plant Genes
Cyclin-Dependent Kinases
computer analysis
DNA
Phase Transition
cyclin-dependent kinase
Restriction Fragment Length Polymorphisms

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa : Chromosomal mapping and expression. / Savouré, Arnould; Fehér, A.; Kaló, P.; Petrovics, György; Csanádi, Gyula; Szecsi, Judit; Kiss, G.; Brown, Spencer; Kondorosi, Adam; Kondorosi, É.

In: Plant Molecular Biology, Vol. 27, No. 6, 03.1995, p. 1059-1070.

Research output: Article

Savouré, Arnould ; Fehér, A. ; Kaló, P. ; Petrovics, György ; Csanádi, Gyula ; Szecsi, Judit ; Kiss, G. ; Brown, Spencer ; Kondorosi, Adam ; Kondorosi, É. / Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa : Chromosomal mapping and expression. In: Plant Molecular Biology. 1995 ; Vol. 27, No. 6. pp. 1059-1070.
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