Isolation and characterization of aniline-degrading Rhodococcus sp. strain AN5

Rensheng Zhuang, Wenhui Zhong, Jun Yao, Huilun Chen, Lin Tian, Yong Zhou, Fei Wang, Emilia Bramanti, G. Záray

Research output: Article

12 Citations (Scopus)

Abstract

A pure culture using aniline as a sole source of carbon and energy was isolated by selective enrichment culturing on a minimum salt medium (MSM) from the Nanjing Chemical Plant, Nanjing, Jiangsu Province, China. An analysis of the 16S rRNA gene sequence and morphological and physiological characteristics showed that this strain was a member of the genus Rhodococcus, and it was designated as strain AN5. Its optimal conditions for aniline biodegradation were 30°C (pH 7.0). Due to the water insolubility of aniline, growth of strain AN5 at various aniline concentrations was studied via spectrophotometry and microcalorimetry in different culture mediums. Strain AN5 not only utilized aniline as its sole carbon and energy resources, but also degraded phenol, benzoic acid and naphthalin. Glucose, peptone and ammonium sulfate, when utilized as its secondary carbon and nitrogen sources, had no active effect on the aniline biodegradation. Such findings would be valuable in the application of strain AN5 to depurate industrial waste water.

Original languageEnglish
Pages (from-to)2009-2016
Number of pages8
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume42
Issue number13
DOIs
Publication statusPublished - nov. 2007

Fingerprint

Aniline
biodegradation
carbon
Carbon
ammonium sulfate
energy resource
spectrophotometry
Biodegradation
phenol
glucose
salt
Industrial Waste
Peptones
Benzoic Acid
Benzoic acid
gene
Chemical plants
acid
nitrogen
Industrial wastes

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Isolation and characterization of aniline-degrading Rhodococcus sp. strain AN5. / Zhuang, Rensheng; Zhong, Wenhui; Yao, Jun; Chen, Huilun; Tian, Lin; Zhou, Yong; Wang, Fei; Bramanti, Emilia; Záray, G.

In: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Vol. 42, No. 13, 11.2007, p. 2009-2016.

Research output: Article

Zhuang, Rensheng ; Zhong, Wenhui ; Yao, Jun ; Chen, Huilun ; Tian, Lin ; Zhou, Yong ; Wang, Fei ; Bramanti, Emilia ; Záray, G. / Isolation and characterization of aniline-degrading Rhodococcus sp. strain AN5. In: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering. 2007 ; Vol. 42, No. 13. pp. 2009-2016.
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