Ischemia/reperfusion-induced changes in intracellular free Ca2+ levels in rat skeletal muscle fibers - An in vivo study

Tamás Ivanics, Zsuzsa Miklós, Zoltán Ruttner, Sándor Bátkai, Dick W. Slaaf, Robert S. Reneman, A. Tóth, László Ligeti

Research output: Article

10 Citations (Scopus)

Abstract

Accumulation of intracellular free calcium (Ca2+(i)) may play an essential role in the ischemia/reperfusion injury of skeletal muscle. Although it has been shown that Ca2+(i) levels significantly increase during ischemia/reperfusion, it is still a matter of debate whether Ca2+(i) increases during ischemia alone. It was the aim of this study to monitor the in vivo Ca2+(i) levels in the rat spinotrapezius muscle during ischemia of varying duration and reperfusion, using a ratiometric fluorescence technique, and to investigate the relationship between the postischemic flow patterns and Ca2+(i), if any. The muscle was loaded with Indo-1/AM and imaged by a cooled digital camera. Pre- and postischemic tissue perfusion was assessed by means of an analogue camera. Our results show that short-term ischemia (5, 15 and 30 min) and subsequent reperfusion (60 min) does not alter Ca2+(i) homeostasis and that tissue perfusion promptly recovers after the insult. One or two hours of ischemia resulted in changes in Ca2+(i) levels, varying from preparation to preparation; increases in some and no changes in others. In these preparations three distinct flow patterns - normal, compromised and no-reflow - could be distinguished during the 60-min reperfusion. Our main conclusion is that in skeletal muscle Ca2+(i) levels may increase, the increase probably depending on the muscle fiber type exposed.

Original languageEnglish
Pages (from-to)302-308
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Volume440
Issue number2
DOIs
Publication statusPublished - 2000

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Skeletal Muscle Fibers
Reperfusion
Muscle
Rats
Ischemia
Fibers
Muscles
Flow patterns
Skeletal Muscle
Perfusion
Tissue
Digital cameras
Reperfusion Injury
Homeostasis
Fluorescence
Cameras
Calcium

ASJC Scopus subject areas

  • Physiology

Cite this

Ischemia/reperfusion-induced changes in intracellular free Ca2+ levels in rat skeletal muscle fibers - An in vivo study. / Ivanics, Tamás; Miklós, Zsuzsa; Ruttner, Zoltán; Bátkai, Sándor; Slaaf, Dick W.; Reneman, Robert S.; Tóth, A.; Ligeti, László.

In: Pflugers Archiv European Journal of Physiology, Vol. 440, No. 2, 2000, p. 302-308.

Research output: Article

Ivanics, Tamás ; Miklós, Zsuzsa ; Ruttner, Zoltán ; Bátkai, Sándor ; Slaaf, Dick W. ; Reneman, Robert S. ; Tóth, A. ; Ligeti, László. / Ischemia/reperfusion-induced changes in intracellular free Ca2+ levels in rat skeletal muscle fibers - An in vivo study. In: Pflugers Archiv European Journal of Physiology. 2000 ; Vol. 440, No. 2. pp. 302-308.
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