Ischemia induces a translocation of the splicing factor tra2-β1 and changes alternative splicing patterns in the brain

Rosette Daoud, Günter Mies, Agata Smialowska, Laszlo Oláh, Konstantin Alexander Hossmann, Stefan Stamm

Research output: Article

74 Citations (Scopus)

Abstract

Alternative splice-site selection is regulated by the relative concentration of individual members of the serine-arginine family of proteins and heterogeneous nuclear ribonucleoproteins. Most of these proteins accumulate predominantly in the nucleus, and a subset of them shuttles continuously between nucleus and cytosol. We demonstrate that in primary neuronal cultures, a rise in intracellular calcium concentration induced by thapsigargin leads to a translocation of the splicing regulatory protein tra2-β1 and a consequent change in splice-site selection. To investigate this phenomenon under physiological conditions, we used an ischemia model. Ischemia induced in the brain causes a cytoplasmic accumulation and hyperphosphorylation of tra2-β1. In addition, several of the proteins binding to tra2-β1, such as src associated in mitosis 68 and serine/arginine-rich proteins, accumulate in the cytosol. Concomitant with this subcellular relocalization, we observed a change in alternative splice-site usage of the ICH-1 gene. The increased usage of its alternative exons is in agreement with previous studies demonstrating its repression by a high concentration of proteins with serine/arginine-rich domains. Our findings suggest that a change in the calcium concentration associated with ischemia is part of a signaling event, which changes pre-mRNA splicing pathways by causing relocalization of proteins that regulate splice-site selection.

Original languageEnglish
Pages (from-to)5889-5899
Number of pages11
JournalJournal of Neuroscience
Volume22
Issue number14
DOIs
Publication statusPublished - júl. 15 2002

ASJC Scopus subject areas

  • Neuroscience(all)

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