Investigation of the putative rate-limiting role of electron transfer in fatty acid desaturation using transfected HEK293T cells

Veronika Zámbó, Laura Simon-Szabó, Farkas Sarnyai, Judit Mátyási, Zsófia Gór-Nagy, Anna Somogyi, Péter Szelényi, Éva Kereszturi, Blanka Tóth, Miklós Csala

Research output: Article

Abstract

Elevated fatty acid (FA) levels contribute to severe metabolic diseases. Unbalanced oversupply of saturated FAs is particularly damaging, which renders stearoyl-CoA desaturase (SCD1) activity an important factor of resistance. A SCD1-related oxidoreductase protects cells against palmitate toxicity, so we aimed to test whether desaturase activity is limited by SCD1 itself or by the associated electron supply. Unsaturated/saturated FA ratio was markedly elevated by SCD1 overexpression while it remained unaffected by the overexpression of SCD1-related electron transfer proteins in HEK293T cells. Electron supply was not rate-limiting either in palmitate-treated cells or in cells with enhanced SCD1 expression. Our findings indicate the rate-limiting role of SCD1 itself, and that FA desaturation cannot be facilitated by reinforcing the electron supply of the enzyme.

Original languageEnglish
Pages (from-to)530-539
Number of pages10
JournalFEBS letters
Volume594
Issue number3
DOIs
Publication statusPublished - febr. 1 2020

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Zámbó, V., Simon-Szabó, L., Sarnyai, F., Mátyási, J., Gór-Nagy, Z., Somogyi, A., Szelényi, P., Kereszturi, É., Tóth, B., & Csala, M. (2020). Investigation of the putative rate-limiting role of electron transfer in fatty acid desaturation using transfected HEK293T cells. FEBS letters, 594(3), 530-539. https://doi.org/10.1002/1873-3468.13622