Investigation of the ecology of francisella tularensis during an inter-epizootic period

Miklós Gyuranecz, Krisztina Rigó, A. Dán, G. Földvári, L. Makrai, B. Dénes, L. Fodor, Gábor Majoros, László Tirják, K. Erdélyi

Research output: Article

47 Citations (Scopus)

Abstract

A 1-year study of the ecological cycle of Francisella tularensis was performed in an enzootic area during an inter-epizootic period. The study was based on multiple sampling of all major constituents of the disease cycle. Seroprevalence of tularemia in the European brown hare (Lepus europaeus) population was 5.1% (10/197) with low antibody titers (1/10 and 1/20), and F. tularensis ssp. holarctica was isolated from four hares. F. tularensis was not detected in the 38 common voles (Microtus arvalis), 110 yellow-necked mice (Apodemus flavicollis), or 15 stripped field mice (Apodemus agrarius) trapped during the study, or the by-catch of 8 Eurasian pygmy shrews (Sorex minutus) or 6 common shrews (Sorex araneus). A total of 1106 Ixodes ricinus and 476 Haemaphysalis concinna ticks were collected from vegetation, and 404 I. ricinus, 28 H. concinna ticks, and 15 Ctenophtalmus assimilis and 10 Nosopsyllus fasciatus fleas were combed off small mammals. One H. concinna female and one nymph collected from the vegetation was found infected with F. tularensis ssp. holarctica by TaqMan polymerase chain reaction, thus resulting a 0.42% (2/476) prevalence. F. tularensis-specific DNA was not detected in environmental water samples, and the examined 100 sheep, 50 cows, and 50 buffalos grazed at the study area were all seronegative. During inter-epizootic periods, F. tularensis ssp. holarctica seems to persist only in the European brown hare-H. concinna cycle at the studied habitat. H. concinna may not serve exclusively as an arthropod vector, but it may also harbor bacteria for 3-4 years through multiple life stages and act as an important reservoir of F. tularensis. Rodent species probably do not serve as true reservoir hosts of tularemia.

Original languageEnglish
Pages (from-to)1031-1035
Number of pages5
JournalVector-Borne and Zoonotic Diseases
Volume11
Issue number8
DOIs
Publication statusPublished - aug. 1 2011

Fingerprint

Francisella tularensis
Ecology
Hares
Shrews
Murinae
Tularemia
Ixodes
Arvicolinae
Ticks
Arthropod Vectors
Siphonaptera
Nymph
Seroepidemiologic Studies
Ecosystem
Mammals
Rodentia
Sheep
Bacteria
Polymerase Chain Reaction
Water

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Virology

Cite this

Investigation of the ecology of francisella tularensis during an inter-epizootic period. / Gyuranecz, Miklós; Rigó, Krisztina; Dán, A.; Földvári, G.; Makrai, L.; Dénes, B.; Fodor, L.; Majoros, Gábor; Tirják, László; Erdélyi, K.

In: Vector-Borne and Zoonotic Diseases, Vol. 11, No. 8, 01.08.2011, p. 1031-1035.

Research output: Article

@article{cc885493f80d43b8bc68941daf7846c1,
title = "Investigation of the ecology of francisella tularensis during an inter-epizootic period",
abstract = "A 1-year study of the ecological cycle of Francisella tularensis was performed in an enzootic area during an inter-epizootic period. The study was based on multiple sampling of all major constituents of the disease cycle. Seroprevalence of tularemia in the European brown hare (Lepus europaeus) population was 5.1{\%} (10/197) with low antibody titers (1/10 and 1/20), and F. tularensis ssp. holarctica was isolated from four hares. F. tularensis was not detected in the 38 common voles (Microtus arvalis), 110 yellow-necked mice (Apodemus flavicollis), or 15 stripped field mice (Apodemus agrarius) trapped during the study, or the by-catch of 8 Eurasian pygmy shrews (Sorex minutus) or 6 common shrews (Sorex araneus). A total of 1106 Ixodes ricinus and 476 Haemaphysalis concinna ticks were collected from vegetation, and 404 I. ricinus, 28 H. concinna ticks, and 15 Ctenophtalmus assimilis and 10 Nosopsyllus fasciatus fleas were combed off small mammals. One H. concinna female and one nymph collected from the vegetation was found infected with F. tularensis ssp. holarctica by TaqMan polymerase chain reaction, thus resulting a 0.42{\%} (2/476) prevalence. F. tularensis-specific DNA was not detected in environmental water samples, and the examined 100 sheep, 50 cows, and 50 buffalos grazed at the study area were all seronegative. During inter-epizootic periods, F. tularensis ssp. holarctica seems to persist only in the European brown hare-H. concinna cycle at the studied habitat. H. concinna may not serve exclusively as an arthropod vector, but it may also harbor bacteria for 3-4 years through multiple life stages and act as an important reservoir of F. tularensis. Rodent species probably do not serve as true reservoir hosts of tularemia.",
keywords = "Disease ecology, European brown hare, Francisella tularensis ssp., Haemaphysalis concinna, holarctica, Rodent, Tick, Tularemia, Zoonosis",
author = "Mikl{\'o}s Gyuranecz and Krisztina Rig{\'o} and A. D{\'a}n and G. F{\"o}ldv{\'a}ri and L. Makrai and B. D{\'e}nes and L. Fodor and G{\'a}bor Majoros and L{\'a}szl{\'o} Tirj{\'a}k and K. Erd{\'e}lyi",
year = "2011",
month = "8",
day = "1",
doi = "10.1089/vbz.2010.0091",
language = "English",
volume = "11",
pages = "1031--1035",
journal = "Vector-Borne and Zoonotic Diseases",
issn = "1530-3667",
publisher = "Mary Ann Liebert Inc.",
number = "8",

}

TY - JOUR

T1 - Investigation of the ecology of francisella tularensis during an inter-epizootic period

AU - Gyuranecz, Miklós

AU - Rigó, Krisztina

AU - Dán, A.

AU - Földvári, G.

AU - Makrai, L.

AU - Dénes, B.

AU - Fodor, L.

AU - Majoros, Gábor

AU - Tirják, László

AU - Erdélyi, K.

PY - 2011/8/1

Y1 - 2011/8/1

N2 - A 1-year study of the ecological cycle of Francisella tularensis was performed in an enzootic area during an inter-epizootic period. The study was based on multiple sampling of all major constituents of the disease cycle. Seroprevalence of tularemia in the European brown hare (Lepus europaeus) population was 5.1% (10/197) with low antibody titers (1/10 and 1/20), and F. tularensis ssp. holarctica was isolated from four hares. F. tularensis was not detected in the 38 common voles (Microtus arvalis), 110 yellow-necked mice (Apodemus flavicollis), or 15 stripped field mice (Apodemus agrarius) trapped during the study, or the by-catch of 8 Eurasian pygmy shrews (Sorex minutus) or 6 common shrews (Sorex araneus). A total of 1106 Ixodes ricinus and 476 Haemaphysalis concinna ticks were collected from vegetation, and 404 I. ricinus, 28 H. concinna ticks, and 15 Ctenophtalmus assimilis and 10 Nosopsyllus fasciatus fleas were combed off small mammals. One H. concinna female and one nymph collected from the vegetation was found infected with F. tularensis ssp. holarctica by TaqMan polymerase chain reaction, thus resulting a 0.42% (2/476) prevalence. F. tularensis-specific DNA was not detected in environmental water samples, and the examined 100 sheep, 50 cows, and 50 buffalos grazed at the study area were all seronegative. During inter-epizootic periods, F. tularensis ssp. holarctica seems to persist only in the European brown hare-H. concinna cycle at the studied habitat. H. concinna may not serve exclusively as an arthropod vector, but it may also harbor bacteria for 3-4 years through multiple life stages and act as an important reservoir of F. tularensis. Rodent species probably do not serve as true reservoir hosts of tularemia.

AB - A 1-year study of the ecological cycle of Francisella tularensis was performed in an enzootic area during an inter-epizootic period. The study was based on multiple sampling of all major constituents of the disease cycle. Seroprevalence of tularemia in the European brown hare (Lepus europaeus) population was 5.1% (10/197) with low antibody titers (1/10 and 1/20), and F. tularensis ssp. holarctica was isolated from four hares. F. tularensis was not detected in the 38 common voles (Microtus arvalis), 110 yellow-necked mice (Apodemus flavicollis), or 15 stripped field mice (Apodemus agrarius) trapped during the study, or the by-catch of 8 Eurasian pygmy shrews (Sorex minutus) or 6 common shrews (Sorex araneus). A total of 1106 Ixodes ricinus and 476 Haemaphysalis concinna ticks were collected from vegetation, and 404 I. ricinus, 28 H. concinna ticks, and 15 Ctenophtalmus assimilis and 10 Nosopsyllus fasciatus fleas were combed off small mammals. One H. concinna female and one nymph collected from the vegetation was found infected with F. tularensis ssp. holarctica by TaqMan polymerase chain reaction, thus resulting a 0.42% (2/476) prevalence. F. tularensis-specific DNA was not detected in environmental water samples, and the examined 100 sheep, 50 cows, and 50 buffalos grazed at the study area were all seronegative. During inter-epizootic periods, F. tularensis ssp. holarctica seems to persist only in the European brown hare-H. concinna cycle at the studied habitat. H. concinna may not serve exclusively as an arthropod vector, but it may also harbor bacteria for 3-4 years through multiple life stages and act as an important reservoir of F. tularensis. Rodent species probably do not serve as true reservoir hosts of tularemia.

KW - Disease ecology

KW - European brown hare

KW - Francisella tularensis ssp.

KW - Haemaphysalis concinna

KW - holarctica

KW - Rodent

KW - Tick

KW - Tularemia

KW - Zoonosis

UR - http://www.scopus.com/inward/record.url?scp=80051517980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051517980&partnerID=8YFLogxK

U2 - 10.1089/vbz.2010.0091

DO - 10.1089/vbz.2010.0091

M3 - Article

C2 - 21142970

AN - SCOPUS:80051517980

VL - 11

SP - 1031

EP - 1035

JO - Vector-Borne and Zoonotic Diseases

JF - Vector-Borne and Zoonotic Diseases

SN - 1530-3667

IS - 8

ER -