Investigation of the conformation of d-glyceraldehyde-3-phosphate dehydrogenase

I. A. Bolotina, D. S. Markovich, M. V. Volkenstein, P. Závodszky

Research output: Article

24 Citations (Scopus)

Abstract

The conformation of d-glyceraldehyde-3-phosphate dehydrogenase was studied by means of spectropolarimetry under various conditions. It was shown that at neutral pH the enzyme possesed a compact globular structure with approx. 40% α-helical content. When the pH was altered, a change in secondary and tertiary structure was observed which was manifested in the change of α-helicity and in the uncoiling of the molecules. Sodium dodecylsulphate produced a change in the tertiary and quaternary structure, i.e. uncoiling of the molecules and the division of the enzyme molecule into subunits, while the secondary structure remained unchanged. The coenzyme (NAD+) and the substrate (glyceraldehyde) produced conformational changes in the apoenzyme molecule. The results can be interpreted in terms of Koshland's "induced-fit" hypothesis.

Original languageEnglish
Pages (from-to)260-270
Number of pages11
JournalBBA - Enzymology
Volume132
Issue number2
Publication statusPublished - márc. 15 1967

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Glyceraldehyde-3-Phosphate Dehydrogenases
Glyceraldehyde
Apoenzymes
Coenzymes
Enzymes
NAD
Sodium

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Bolotina, I. A., Markovich, D. S., Volkenstein, M. V., & Závodszky, P. (1967). Investigation of the conformation of d-glyceraldehyde-3-phosphate dehydrogenase. BBA - Enzymology, 132(2), 260-270.

Investigation of the conformation of d-glyceraldehyde-3-phosphate dehydrogenase. / Bolotina, I. A.; Markovich, D. S.; Volkenstein, M. V.; Závodszky, P.

In: BBA - Enzymology, Vol. 132, No. 2, 15.03.1967, p. 260-270.

Research output: Article

Bolotina, IA, Markovich, DS, Volkenstein, MV & Závodszky, P 1967, 'Investigation of the conformation of d-glyceraldehyde-3-phosphate dehydrogenase', BBA - Enzymology, vol. 132, no. 2, pp. 260-270.
Bolotina IA, Markovich DS, Volkenstein MV, Závodszky P. Investigation of the conformation of d-glyceraldehyde-3-phosphate dehydrogenase. BBA - Enzymology. 1967 márc. 15;132(2):260-270.
Bolotina, I. A. ; Markovich, D. S. ; Volkenstein, M. V. ; Závodszky, P. / Investigation of the conformation of d-glyceraldehyde-3-phosphate dehydrogenase. In: BBA - Enzymology. 1967 ; Vol. 132, No. 2. pp. 260-270.
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