Investigation of iron pools in cucumber roots by Mössbauer spectroscopy

Direct evidence for the Strategy I iron uptake mechanism

Krisztina Kovács, E. Kuzmann, E. Tatár, A. Vértes, F. Fodor

Research output: Article

13 Citations (Scopus)

Abstract

Distinct chemical species of iron were investigated by Mössbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. Mössbauer spectra of iron deficient roots supplied with 10-500 μM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The Mössbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No Fe II was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of Fe II-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalPlanta
Volume229
Issue number2
DOIs
Publication statusPublished - jan. 2009

Fingerprint

Cucumis sativus
cucumbers
spectroscopy
Spectrum Analysis
Iron
iron
uptake mechanisms
Citric Acid
citrates
Oxidoreductases
ferric sulfate
chemical speciation
hydroxides
nutrient solutions
Cytoplasm
cytoplasm

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

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title = "Investigation of iron pools in cucumber roots by M{\"o}ssbauer spectroscopy: Direct evidence for the Strategy I iron uptake mechanism",
abstract = "Distinct chemical species of iron were investigated by M{\"o}ssbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. M{\"o}ssbauer spectra of iron deficient roots supplied with 10-500 μM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The M{\"o}ssbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No Fe II was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of Fe II-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.",
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T1 - Investigation of iron pools in cucumber roots by Mössbauer spectroscopy

T2 - Direct evidence for the Strategy I iron uptake mechanism

AU - Kovács, Krisztina

AU - Kuzmann, E.

AU - Tatár, E.

AU - Vértes, A.

AU - Fodor, F.

PY - 2009/1

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AB - Distinct chemical species of iron were investigated by Mössbauer spectroscopy during iron uptake into cucumber roots grown in unbuffered nutrient solution with or without 57Fe-citrate. Mössbauer spectra of iron deficient roots supplied with 10-500 μM 57Fe-citrate for 30-180 min and 24 h and iron-sufficient ones, were recorded. The roots were analysed for Fe concentration and Fe reductase activity. The Mössbauer parameters in the case of iron-sufficient roots revealed high-spin iron(III) components suggesting the presence of FeIII-carboxylate complexes, hydrous ferric oxides and sulfate-hydroxide containing species. No Fe II was detected in these roots. However, iron-deficient roots supplied with 0.5 mM 57FeIII-citrate for 30 min contained significant amount of FeII in a hexaaqua complex form. This is a direct evidence for the Strategy I iron uptake mechanism. Correlation was found between the decrease in Fe reductase activity and the ratio of Fe II-FeIII components as the time of iron supply was increased. The data may refer to a higher iron reduction rate as compared to its uptake/reoxidation in the cytoplasm in accordance with the increased reduction rate in iron deficient Strategy I plants.

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KW - Mössbauer spectroscopy

KW - Strategy I

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