Investigation of catechol 2,3-dioxygenase and 16S rRNA gene diversity in hypoxic, petroleum hydrocarbon contaminated groundwater

András Táncsics, István Szabó, Erzsébet Baka, Sándor Szoboszlay, József Kukolya, Balázs Kriszt, Károly Márialigeti

Research output: Article

30 Citations (Scopus)

Abstract

Detection of catechol 2,3-dioxygenase genes in aromatic hydrocarbon contaminated environments gives the opportunity to measure the diversity of bacteria involved in the degradation of the contaminants under aerobic conditions. In this study, we investigated the diversity and distribution of Comamonadaceae family (Betaproteobacteria) related catechol 2,3-dioxygenase genes, which belong to the I.2.C subfamily of extradiol dioxygenase genes. These catabolic genes encode enzymes supposed to function under hypoxic conditions as well, and may play a notable role in BTEX degradation in oxygen limited environments. Therefore, their diversity was analyzed in oxygen limited, petroleum hydrocarbon contaminated groundwater by terminal restriction fragment length polymorphism and cloning. Subfamily I.2.C related catechol 2,3-dioxygenase genes were detected in every investigated groundwater sample and a dynamic change was observed in the case of the structure of C23O gene possessing bacterial communities. To link the metabolic capability to the microbial structure, 16S rRNA gene-based clone libraries were generated and it was concluded that Betaproteobacteria were abundant in the bacterial communities of the contaminated samples. These results support the opinion that Betaproteobacteria may play a significant role in BTEX degradation under hypoxic conditions.

Original languageEnglish
Pages (from-to)398-406
Number of pages9
JournalSystematic and Applied Microbiology
Volume33
Issue number7
DOIs
Publication statusPublished - nov. 2010

Fingerprint

Catechol 2,3-Dioxygenase
catechol
Groundwater
Petroleum
petroleum hydrocarbon
Hydrocarbons
rRNA Genes
petroleum
hydrocarbons
Betaproteobacteria
groundwater
ribosomal RNA
gene
beta-Proteobacteria
Genes
genes
BTEX
Comamonadaceae
bacterial communities
Oxygen

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

Investigation of catechol 2,3-dioxygenase and 16S rRNA gene diversity in hypoxic, petroleum hydrocarbon contaminated groundwater. / Táncsics, András; Szabó, István; Baka, Erzsébet; Szoboszlay, Sándor; Kukolya, József; Kriszt, Balázs; Márialigeti, Károly.

In: Systematic and Applied Microbiology, Vol. 33, No. 7, 11.2010, p. 398-406.

Research output: Article

Táncsics, András ; Szabó, István ; Baka, Erzsébet ; Szoboszlay, Sándor ; Kukolya, József ; Kriszt, Balázs ; Márialigeti, Károly. / Investigation of catechol 2,3-dioxygenase and 16S rRNA gene diversity in hypoxic, petroleum hydrocarbon contaminated groundwater. In: Systematic and Applied Microbiology. 2010 ; Vol. 33, No. 7. pp. 398-406.
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