Investigation of allergen proteins in five tomato cultivars

R. T. Mócsai, A. MacZó, C. Grünwald-Gruber, K. Majer-Baranyi, N. Adányi, P. Milotay, J. Czelecz, R. Tömösközi-Farkas

Research output: Article

Abstract

Investigation of putative allergens from tomato berries is challenging as differences between human serum IgE specificity and reactivity as well as the non-specific binding of the primary and secondary antibodies often cause difficulties. In this study five tomato cultivars were investigated to evaluate their potential allergenicity in Hungarian tomato sensitive patients. The major allergens proved to be low molecular weight proteins, but several previously described allergens could be identified as well using IgE-Western blotting. IgE binding to cross-reactive carbohydrate determinants (CCDs) was ruled out through the use of CCD inhibitor, but non-specific binding of the secondary antibody remained an issue. IgE binding activity of a purified, immunoblot positive protein (band at 40 kDa), and non-specific binding of the secondary antibody to the same protein, was demonstrated with an Optical Waveguide Lightmode Spectroscopy (OWLS) based immunosensor. LC-ESI-MS/MS analysis showed this protein is an as-yet undescribed vicilin-type putative allergen.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalActa Alimentaria
Volume46
Issue number3
DOIs
Publication statusPublished - szept. 1 2017

Fingerprint

Lycopersicon esculentum
allergens
Allergens
Immunoglobulin E
tomatoes
cultivars
antibodies
Antibodies
Proteins
proteins
Carbohydrates
carbohydrates
vicilin
allergenicity
small fruits
spectroscopy
Fruit
Spectrum Analysis
Western blotting
Molecular Weight

ASJC Scopus subject areas

  • Food Science

Cite this

Mócsai, R. T., MacZó, A., Grünwald-Gruber, C., Majer-Baranyi, K., Adányi, N., Milotay, P., ... Tömösközi-Farkas, R. (2017). Investigation of allergen proteins in five tomato cultivars. Acta Alimentaria, 46(3), 369-377. https://doi.org/10.1556/066.2017.46.3.13

Investigation of allergen proteins in five tomato cultivars. / Mócsai, R. T.; MacZó, A.; Grünwald-Gruber, C.; Majer-Baranyi, K.; Adányi, N.; Milotay, P.; Czelecz, J.; Tömösközi-Farkas, R.

In: Acta Alimentaria, Vol. 46, No. 3, 01.09.2017, p. 369-377.

Research output: Article

Mócsai, RT, MacZó, A, Grünwald-Gruber, C, Majer-Baranyi, K, Adányi, N, Milotay, P, Czelecz, J & Tömösközi-Farkas, R 2017, 'Investigation of allergen proteins in five tomato cultivars', Acta Alimentaria, vol. 46, no. 3, pp. 369-377. https://doi.org/10.1556/066.2017.46.3.13
Mócsai RT, MacZó A, Grünwald-Gruber C, Majer-Baranyi K, Adányi N, Milotay P et al. Investigation of allergen proteins in five tomato cultivars. Acta Alimentaria. 2017 szept. 1;46(3):369-377. https://doi.org/10.1556/066.2017.46.3.13
Mócsai, R. T. ; MacZó, A. ; Grünwald-Gruber, C. ; Majer-Baranyi, K. ; Adányi, N. ; Milotay, P. ; Czelecz, J. ; Tömösközi-Farkas, R. / Investigation of allergen proteins in five tomato cultivars. In: Acta Alimentaria. 2017 ; Vol. 46, No. 3. pp. 369-377.
@article{c15a77d32f6c420b939453501aefa41c,
title = "Investigation of allergen proteins in five tomato cultivars",
abstract = "Investigation of putative allergens from tomato berries is challenging as differences between human serum IgE specificity and reactivity as well as the non-specific binding of the primary and secondary antibodies often cause difficulties. In this study five tomato cultivars were investigated to evaluate their potential allergenicity in Hungarian tomato sensitive patients. The major allergens proved to be low molecular weight proteins, but several previously described allergens could be identified as well using IgE-Western blotting. IgE binding to cross-reactive carbohydrate determinants (CCDs) was ruled out through the use of CCD inhibitor, but non-specific binding of the secondary antibody remained an issue. IgE binding activity of a purified, immunoblot positive protein (band at 40 kDa), and non-specific binding of the secondary antibody to the same protein, was demonstrated with an Optical Waveguide Lightmode Spectroscopy (OWLS) based immunosensor. LC-ESI-MS/MS analysis showed this protein is an as-yet undescribed vicilin-type putative allergen.",
keywords = "IgE non-specific binding, Immunosensor, LC-ESI-MS/MS, Tomato allergens",
author = "M{\'o}csai, {R. T.} and A. MacZ{\'o} and C. Gr{\"u}nwald-Gruber and K. Majer-Baranyi and N. Ad{\'a}nyi and P. Milotay and J. Czelecz and R. T{\"o}m{\"o}sk{\"o}zi-Farkas",
year = "2017",
month = "9",
day = "1",
doi = "10.1556/066.2017.46.3.13",
language = "English",
volume = "46",
pages = "369--377",
journal = "Acta Alimentaria",
issn = "0139-3006",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Investigation of allergen proteins in five tomato cultivars

AU - Mócsai, R. T.

AU - MacZó, A.

AU - Grünwald-Gruber, C.

AU - Majer-Baranyi, K.

AU - Adányi, N.

AU - Milotay, P.

AU - Czelecz, J.

AU - Tömösközi-Farkas, R.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Investigation of putative allergens from tomato berries is challenging as differences between human serum IgE specificity and reactivity as well as the non-specific binding of the primary and secondary antibodies often cause difficulties. In this study five tomato cultivars were investigated to evaluate their potential allergenicity in Hungarian tomato sensitive patients. The major allergens proved to be low molecular weight proteins, but several previously described allergens could be identified as well using IgE-Western blotting. IgE binding to cross-reactive carbohydrate determinants (CCDs) was ruled out through the use of CCD inhibitor, but non-specific binding of the secondary antibody remained an issue. IgE binding activity of a purified, immunoblot positive protein (band at 40 kDa), and non-specific binding of the secondary antibody to the same protein, was demonstrated with an Optical Waveguide Lightmode Spectroscopy (OWLS) based immunosensor. LC-ESI-MS/MS analysis showed this protein is an as-yet undescribed vicilin-type putative allergen.

AB - Investigation of putative allergens from tomato berries is challenging as differences between human serum IgE specificity and reactivity as well as the non-specific binding of the primary and secondary antibodies often cause difficulties. In this study five tomato cultivars were investigated to evaluate their potential allergenicity in Hungarian tomato sensitive patients. The major allergens proved to be low molecular weight proteins, but several previously described allergens could be identified as well using IgE-Western blotting. IgE binding to cross-reactive carbohydrate determinants (CCDs) was ruled out through the use of CCD inhibitor, but non-specific binding of the secondary antibody remained an issue. IgE binding activity of a purified, immunoblot positive protein (band at 40 kDa), and non-specific binding of the secondary antibody to the same protein, was demonstrated with an Optical Waveguide Lightmode Spectroscopy (OWLS) based immunosensor. LC-ESI-MS/MS analysis showed this protein is an as-yet undescribed vicilin-type putative allergen.

KW - IgE non-specific binding

KW - Immunosensor

KW - LC-ESI-MS/MS

KW - Tomato allergens

UR - http://www.scopus.com/inward/record.url?scp=85027099236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027099236&partnerID=8YFLogxK

U2 - 10.1556/066.2017.46.3.13

DO - 10.1556/066.2017.46.3.13

M3 - Article

AN - SCOPUS:85027099236

VL - 46

SP - 369

EP - 377

JO - Acta Alimentaria

JF - Acta Alimentaria

SN - 0139-3006

IS - 3

ER -