Intranasal delivery of human β-amyloid peptide in rats: Effective brain targeting

Eszter Sipos, Anita Kurunczi, András Fehér, Zsuzsa Penke, Lívia Fülöp, Ágnes Kasza, János Horváth, Sándor Horvát, Szilvia Veszelka, Gábor Balogh, Levente Kürti, István Eros, Piroska Szabó-Révész, Árpád Párducz, Botond Penke, Mária A. Deli

Research output: Article

23 Citations (Scopus)

Abstract

(1) Intranasal administration is a non-invasive and effective way for the delivery of drugs to brain that circumvents the blood-brain barrier. The aims of the study were to test a nasal delivery system for human β-amyloid (Aβ) peptides, to measure the delivery of the peptides to brain regions, and to test their biological activity in rats. (2) Aβ1-42, in the form of a mixture of oligomers, protofibrils, and fibrils was dissolved in a nasal formulation containing hydrophobic, hydrophylic, and mucoadhesive components. The peptide solution was administered intranasally to rats as a single dose or in repeated doses. (3) Nasally injected Aβ labeled with the blue fluorescent dye amino-methyl coumarinyl acetic acid (AMCA) could be detected by fluorescent microscopy in the olfactory bulb and frontal cortex. The concentration of the peptide was quantified by fluorescent spectroscopy, and a significant amount of AMCA-Aβ peptide could be detected in the olfactory bulb. Unlabeled Aβ also reached the olfactory bulb and frontal cortex of rats as evidenced by intense immunostaining. (4) In behavioral experiments, nasal Aβ treatment did not affect anxiety levels (open-field test) and short-term memory (Y-maze test), but significantly impaired long-term spatial memory in the Morris water maze. The treatments did not result in Aβ immunization. (5) The tested intranasal delivery system could successfully target a bioactive peptide into the central nervous system and provides a basis for developing a non-invasive and cost effective, new model to study amyloid-induced dysfunctions in the brain.

Original languageEnglish
Pages (from-to)405-413
Number of pages9
JournalCellular and Molecular Neurobiology
Volume30
Issue number3
DOIs
Publication statusPublished - ápr. 1 2010

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ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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