Intracytoplasmic sperm injection (Icsi): The brussels experience

Herman Tournaye, Jian Liu, Zsolt Nagy, Hubert Joris, Ari Wisanto, Maryse Bonduelle, Josiane Van Der Elst, Catherine Staessen, Johan Smitz, Sherman Silber, Paul Devroey, Inge Liebaers, André Van Steirteghem

Research output: Article

54 Citations (Scopus)


The present report covers the results of a 26-month period in which 1275 consecutive treatment cycles by intracytoplasmic sperm injection (ICSI) were performed in 919 couples. These couples were afflicted with male factor infertility and had had at least one previous failed conventional in vitro fertilization (IVF) treatment cycle. In other couples, the husband had semen parameters incompatible with conventional IVF or suffered from excretory azoospermia which required microsurgical epididymal sperm aspiration or testicular sperm retrieval. Overall, the 2 pronuclear (PN) fertilization rate was 47-7% per retrieved oocyte-cumulus complex and 66-4% per successfully injected metaphase II oocyte. Embryo transfer was performed in 90-8% of started cycles and 362 clinical pregnancies were recorded, giving a clinical pregnancy rate of 28-4% per started cycle or 31-3% per transfer. In addition, updated results on the outcome of pregnancies after microasslsted fertilization are presented. As of 30 August 1994, 416 children have been bom. Although 16 major congenital malformations have been observed (3 • 9%), there appears to be no reason for serious concern as regards the occurrence of major congenital anomalies after ICSI.

Original languageEnglish
Pages (from-to)269-279
Number of pages11
JournalReproduction, Fertility and Development
Issue number2
Publication statusPublished - jan. 1 1995


ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Tournaye, H., Liu, J., Nagy, Z., Joris, H., Wisanto, A., Bonduelle, M., Van Der Elst, J., Staessen, C., Smitz, J., Silber, S., Devroey, P., Liebaers, I., & Steirteghem, A. V. (1995). Intracytoplasmic sperm injection (Icsi): The brussels experience. Reproduction, Fertility and Development, 7(2), 269-279.