Intestinal ischemic preconditioning in rats and NF-κB activation

A. Ferencz, B. Rácz, B. Gasz, Laszlo Benko, Gabor Jancso, Maria Kurthy, E. Rőth

Research output: Article

18 Citations (Scopus)

Abstract

Cold preservation prior to small-bowel transplantation can moderate tissue injury, although it is unable to protect the bowel graft from acute reperfusion injury. One method to reduce oxidative stress is ischemic preconditioning (IPC). The limited data regarding IPC of the bowel encouraged us to investigate the key factor in this process, i.e., the activation of nuclear factor-kappa binding (NF-kB) in bowel tissue. The intestine was preconditioned by various cycles of IPC on rats. Activation of NF-kB was detected by a chemiluminescence-based ELISA method. Our findings showed that NF-kB level was elevated significantly 30 min after IPC. One hour following IPC, NF-kB decreased to control level; 2 h after IPC, the level significantly increased again. These changes were independent of the number of IPC cycles. Our experiments with various IPC cycles revealed that even a very short IPC cycle was able to activate the IPC cascade in small-bowel tissue.

Original languageEnglish
Pages (from-to)54-57
Number of pages4
JournalMicrosurgery
Volume26
Issue number1
DOIs
Publication statusPublished - 2006

Fingerprint

Ischemic Preconditioning
Luminescence
Reperfusion Injury
Intestines
Oxidative Stress
Transplantation
Enzyme-Linked Immunosorbent Assay
Transplants

ASJC Scopus subject areas

  • Surgery

Cite this

Intestinal ischemic preconditioning in rats and NF-κB activation. / Ferencz, A.; Rácz, B.; Gasz, B.; Benko, Laszlo; Jancso, Gabor; Kurthy, Maria; Rőth, E.

In: Microsurgery, Vol. 26, No. 1, 2006, p. 54-57.

Research output: Article

Ferencz, A. ; Rácz, B. ; Gasz, B. ; Benko, Laszlo ; Jancso, Gabor ; Kurthy, Maria ; Rőth, E. / Intestinal ischemic preconditioning in rats and NF-κB activation. In: Microsurgery. 2006 ; Vol. 26, No. 1. pp. 54-57.
@article{6696e635dbf14643b66bab353a5da147,
title = "Intestinal ischemic preconditioning in rats and NF-κB activation",
abstract = "Cold preservation prior to small-bowel transplantation can moderate tissue injury, although it is unable to protect the bowel graft from acute reperfusion injury. One method to reduce oxidative stress is ischemic preconditioning (IPC). The limited data regarding IPC of the bowel encouraged us to investigate the key factor in this process, i.e., the activation of nuclear factor-kappa binding (NF-kB) in bowel tissue. The intestine was preconditioned by various cycles of IPC on rats. Activation of NF-kB was detected by a chemiluminescence-based ELISA method. Our findings showed that NF-kB level was elevated significantly 30 min after IPC. One hour following IPC, NF-kB decreased to control level; 2 h after IPC, the level significantly increased again. These changes were independent of the number of IPC cycles. Our experiments with various IPC cycles revealed that even a very short IPC cycle was able to activate the IPC cascade in small-bowel tissue.",
author = "A. Ferencz and B. R{\'a}cz and B. Gasz and Laszlo Benko and Gabor Jancso and Maria Kurthy and E. Rőth",
year = "2006",
doi = "10.1002/micr.20211",
language = "English",
volume = "26",
pages = "54--57",
journal = "Microsurgery",
issn = "0738-1085",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Intestinal ischemic preconditioning in rats and NF-κB activation

AU - Ferencz, A.

AU - Rácz, B.

AU - Gasz, B.

AU - Benko, Laszlo

AU - Jancso, Gabor

AU - Kurthy, Maria

AU - Rőth, E.

PY - 2006

Y1 - 2006

N2 - Cold preservation prior to small-bowel transplantation can moderate tissue injury, although it is unable to protect the bowel graft from acute reperfusion injury. One method to reduce oxidative stress is ischemic preconditioning (IPC). The limited data regarding IPC of the bowel encouraged us to investigate the key factor in this process, i.e., the activation of nuclear factor-kappa binding (NF-kB) in bowel tissue. The intestine was preconditioned by various cycles of IPC on rats. Activation of NF-kB was detected by a chemiluminescence-based ELISA method. Our findings showed that NF-kB level was elevated significantly 30 min after IPC. One hour following IPC, NF-kB decreased to control level; 2 h after IPC, the level significantly increased again. These changes were independent of the number of IPC cycles. Our experiments with various IPC cycles revealed that even a very short IPC cycle was able to activate the IPC cascade in small-bowel tissue.

AB - Cold preservation prior to small-bowel transplantation can moderate tissue injury, although it is unable to protect the bowel graft from acute reperfusion injury. One method to reduce oxidative stress is ischemic preconditioning (IPC). The limited data regarding IPC of the bowel encouraged us to investigate the key factor in this process, i.e., the activation of nuclear factor-kappa binding (NF-kB) in bowel tissue. The intestine was preconditioned by various cycles of IPC on rats. Activation of NF-kB was detected by a chemiluminescence-based ELISA method. Our findings showed that NF-kB level was elevated significantly 30 min after IPC. One hour following IPC, NF-kB decreased to control level; 2 h after IPC, the level significantly increased again. These changes were independent of the number of IPC cycles. Our experiments with various IPC cycles revealed that even a very short IPC cycle was able to activate the IPC cascade in small-bowel tissue.

UR - http://www.scopus.com/inward/record.url?scp=32644447368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32644447368&partnerID=8YFLogxK

U2 - 10.1002/micr.20211

DO - 10.1002/micr.20211

M3 - Article

VL - 26

SP - 54

EP - 57

JO - Microsurgery

JF - Microsurgery

SN - 0738-1085

IS - 1

ER -