Interference in quorum sensing signal transmission amongst microbial species

Zoltán Varga, Mira Szabó, Monika Kerényi, J. Molnár

Research output: Article

3 Citations (Scopus)

Abstract

Antibiotics are usually studied on pure cultures of a single bacterial strain, whereas multi-species communities that inhabit human niches and the biosphere are generally ignored. The modification of quorum sensing (QS) is investigated in a system involving a co-existing signal producer and sensor bacterial cells. A pure culture of merely one bacterial species is quite rare in any niche. The interactions of different bacterial species may therefore be of special importance in pathogenicity, antibiotic resistance and signal transmission.In the present study the authors investigated the QS in model experiments involving several Gram-positive and Gram-negative bacterial species isolated from human infections or laboratory strains. The effects of various compounds on QS were studied in mixed bacterial populations during the incubation period of 24-48 h. As the simplest example of co-existing cell populations, the N-acyl homoserine lactone producing Ezf 10-17 was applied with Chromobacterium violaceum 026 as sensor.The signal of QS transmission between the co-existing QS system and pathogenic bacteria isolated from various patients was found to be modified by certain bacterial cells. The bacterial-bacterial interactions in a mixed flora can change the classical signal transmission in the microbial community and should therefore be taken into consideration in rational chemotherapy.

Original languageEnglish
Pages (from-to)475-484
Number of pages10
JournalActa Microbiologica et Immunologica Hungarica
Volume59
Issue number4
DOIs
Publication statusPublished - dec. 1 2012

Fingerprint

Quorum Sensing
Chromobacterium
Acyl-Butyrolactones
Laboratory Infection
Microbial Drug Resistance
Population
Virulence
Anti-Bacterial Agents
Bacteria
Drug Therapy

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Interference in quorum sensing signal transmission amongst microbial species. / Varga, Zoltán; Szabó, Mira; Kerényi, Monika; Molnár, J.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 59, No. 4, 01.12.2012, p. 475-484.

Research output: Article

Varga, Zoltán ; Szabó, Mira ; Kerényi, Monika ; Molnár, J. / Interference in quorum sensing signal transmission amongst microbial species. In: Acta Microbiologica et Immunologica Hungarica. 2012 ; Vol. 59, No. 4. pp. 475-484.
@article{fa4386242af0493fbf9527f77e48d2f4,
title = "Interference in quorum sensing signal transmission amongst microbial species",
abstract = "Antibiotics are usually studied on pure cultures of a single bacterial strain, whereas multi-species communities that inhabit human niches and the biosphere are generally ignored. The modification of quorum sensing (QS) is investigated in a system involving a co-existing signal producer and sensor bacterial cells. A pure culture of merely one bacterial species is quite rare in any niche. The interactions of different bacterial species may therefore be of special importance in pathogenicity, antibiotic resistance and signal transmission.In the present study the authors investigated the QS in model experiments involving several Gram-positive and Gram-negative bacterial species isolated from human infections or laboratory strains. The effects of various compounds on QS were studied in mixed bacterial populations during the incubation period of 24-48 h. As the simplest example of co-existing cell populations, the N-acyl homoserine lactone producing Ezf 10-17 was applied with Chromobacterium violaceum 026 as sensor.The signal of QS transmission between the co-existing QS system and pathogenic bacteria isolated from various patients was found to be modified by certain bacterial cells. The bacterial-bacterial interactions in a mixed flora can change the classical signal transmission in the microbial community and should therefore be taken into consideration in rational chemotherapy.",
keywords = "Chromobacterium violaceum CV026, coexistence, Escherichia coli, Quorum quenching, Quorum sensing",
author = "Zolt{\'a}n Varga and Mira Szab{\'o} and Monika Ker{\'e}nyi and J. Moln{\'a}r",
year = "2012",
month = "12",
day = "1",
doi = "10.1556/AMicr.59.2012.4.5",
language = "English",
volume = "59",
pages = "475--484",
journal = "Acta Microbiologica et Immunologica Hungarica",
issn = "1217-8950",
publisher = "Akademiai Kiado",
number = "4",

}

TY - JOUR

T1 - Interference in quorum sensing signal transmission amongst microbial species

AU - Varga, Zoltán

AU - Szabó, Mira

AU - Kerényi, Monika

AU - Molnár, J.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Antibiotics are usually studied on pure cultures of a single bacterial strain, whereas multi-species communities that inhabit human niches and the biosphere are generally ignored. The modification of quorum sensing (QS) is investigated in a system involving a co-existing signal producer and sensor bacterial cells. A pure culture of merely one bacterial species is quite rare in any niche. The interactions of different bacterial species may therefore be of special importance in pathogenicity, antibiotic resistance and signal transmission.In the present study the authors investigated the QS in model experiments involving several Gram-positive and Gram-negative bacterial species isolated from human infections or laboratory strains. The effects of various compounds on QS were studied in mixed bacterial populations during the incubation period of 24-48 h. As the simplest example of co-existing cell populations, the N-acyl homoserine lactone producing Ezf 10-17 was applied with Chromobacterium violaceum 026 as sensor.The signal of QS transmission between the co-existing QS system and pathogenic bacteria isolated from various patients was found to be modified by certain bacterial cells. The bacterial-bacterial interactions in a mixed flora can change the classical signal transmission in the microbial community and should therefore be taken into consideration in rational chemotherapy.

AB - Antibiotics are usually studied on pure cultures of a single bacterial strain, whereas multi-species communities that inhabit human niches and the biosphere are generally ignored. The modification of quorum sensing (QS) is investigated in a system involving a co-existing signal producer and sensor bacterial cells. A pure culture of merely one bacterial species is quite rare in any niche. The interactions of different bacterial species may therefore be of special importance in pathogenicity, antibiotic resistance and signal transmission.In the present study the authors investigated the QS in model experiments involving several Gram-positive and Gram-negative bacterial species isolated from human infections or laboratory strains. The effects of various compounds on QS were studied in mixed bacterial populations during the incubation period of 24-48 h. As the simplest example of co-existing cell populations, the N-acyl homoserine lactone producing Ezf 10-17 was applied with Chromobacterium violaceum 026 as sensor.The signal of QS transmission between the co-existing QS system and pathogenic bacteria isolated from various patients was found to be modified by certain bacterial cells. The bacterial-bacterial interactions in a mixed flora can change the classical signal transmission in the microbial community and should therefore be taken into consideration in rational chemotherapy.

KW - Chromobacterium violaceum CV026

KW - coexistence

KW - Escherichia coli

KW - Quorum quenching

KW - Quorum sensing

UR - http://www.scopus.com/inward/record.url?scp=84870595113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870595113&partnerID=8YFLogxK

U2 - 10.1556/AMicr.59.2012.4.5

DO - 10.1556/AMicr.59.2012.4.5

M3 - Article

C2 - 23195556

AN - SCOPUS:84870595113

VL - 59

SP - 475

EP - 484

JO - Acta Microbiologica et Immunologica Hungarica

JF - Acta Microbiologica et Immunologica Hungarica

SN - 1217-8950

IS - 4

ER -