Interaction of hydroxypropyl-β-cyclodextrin with peptides, studied by reversed-phase thin-layer chromatography

E. Gere-Pászti, T. Cserháti, E. Forgács, Z. Deyl, I. Miksik, A. Eckhardt, Z. Illés

Research output: Article

6 Citations (Scopus)

Abstract

The effect of various concentrations of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the reversed-phase thin-layer chromatographic mobility of some low molecular mass peptides was measured in distilled water and in 0.05 M of LiCl, NaCl, KCl, RbCl, and CsCl solutions, and the relative strength of HP-β-CD-peptide interaction has been calculated from the dependence of retention on the concentration of HP-β-CD in the mobile phase. Lipophilicity of the peptides decreased regularly with increasing concentration of HP-β-CD in the mobile phase, proving the interaction (probably the formation of inclusion complexes) between peptides and HP-β-CD. Principal component analysis indicated that each salt influenced the interaction differently. Peptides formed clusters on the two-dimensional nonlinear map according to the character of the amino acid monomer and not according to the number of amino acid units, suggesting that only one terminal amino acid is included in the cavity of HP-β-CD.

Original languageEnglish
Pages (from-to)2619-2632
Number of pages14
JournalJournal of Liquid Chromatography and Related Technologies
Volume28
Issue number16
DOIs
Publication statusPublished - 2005

Fingerprint

Thin layer chromatography
Cyclodextrins
Reverse-Phase Chromatography
Thin Layer Chromatography
Peptides
Amino Acids
Molecular mass
Principal Component Analysis
Principal component analysis
Salts
Monomers
Water

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Interaction of hydroxypropyl-β-cyclodextrin with peptides, studied by reversed-phase thin-layer chromatography. / Gere-Pászti, E.; Cserháti, T.; Forgács, E.; Deyl, Z.; Miksik, I.; Eckhardt, A.; Illés, Z.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 28, No. 16, 2005, p. 2619-2632.

Research output: Article

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AU - Gere-Pászti, E.

AU - Cserháti, T.

AU - Forgács, E.

AU - Deyl, Z.

AU - Miksik, I.

AU - Eckhardt, A.

AU - Illés, Z.

PY - 2005

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N2 - The effect of various concentrations of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the reversed-phase thin-layer chromatographic mobility of some low molecular mass peptides was measured in distilled water and in 0.05 M of LiCl, NaCl, KCl, RbCl, and CsCl solutions, and the relative strength of HP-β-CD-peptide interaction has been calculated from the dependence of retention on the concentration of HP-β-CD in the mobile phase. Lipophilicity of the peptides decreased regularly with increasing concentration of HP-β-CD in the mobile phase, proving the interaction (probably the formation of inclusion complexes) between peptides and HP-β-CD. Principal component analysis indicated that each salt influenced the interaction differently. Peptides formed clusters on the two-dimensional nonlinear map according to the character of the amino acid monomer and not according to the number of amino acid units, suggesting that only one terminal amino acid is included in the cavity of HP-β-CD.

AB - The effect of various concentrations of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the reversed-phase thin-layer chromatographic mobility of some low molecular mass peptides was measured in distilled water and in 0.05 M of LiCl, NaCl, KCl, RbCl, and CsCl solutions, and the relative strength of HP-β-CD-peptide interaction has been calculated from the dependence of retention on the concentration of HP-β-CD in the mobile phase. Lipophilicity of the peptides decreased regularly with increasing concentration of HP-β-CD in the mobile phase, proving the interaction (probably the formation of inclusion complexes) between peptides and HP-β-CD. Principal component analysis indicated that each salt influenced the interaction differently. Peptides formed clusters on the two-dimensional nonlinear map according to the character of the amino acid monomer and not according to the number of amino acid units, suggesting that only one terminal amino acid is included in the cavity of HP-β-CD.

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KW - Nonlinear map

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KW - TLC

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