Integrated evolutionary analysis reveals antimicrobial peptides with limited resistance

Réka Spohn, Lejla Daruka, Viktória Lázár, Ana Martins, Fanni Vidovics, Gábor Grézal, Orsolya Méhi, Bálint Kintses, Mónika Számel, Pramod K. Jangir, Bálint Csörgő, Ádám Györkei, Zoltán Bódi, Anikó Faragó, L. Bodai, I. Földesi, Diána Kata, Gergely Maróti, Bernadett Pap, Roland WirthB. Papp, C. Pál

Research output: Article

Abstract

Antimicrobial peptides (AMPs) are promising antimicrobials, however, the potential of bacterial resistance is a major concern. Here we systematically study the evolution of resistance to 14 chemically diverse AMPs and 12 antibiotics in Escherichia coli. Our work indicates that evolution of resistance against certain AMPs, such as tachyplesin II and cecropin P1, is limited. Resistance level provided by point mutations and gene amplification is very low and antibiotic-resistant bacteria display no cross-resistance to these AMPs. Moreover, genomic fragments derived from a wide range of soil bacteria confer no detectable resistance against these AMPs when introduced into native host bacteria on plasmids. We have found that simple physicochemical features dictate bacterial propensity to evolve resistance against AMPs. Our work could serve as a promising source for the development of new AMP-based therapeutics less prone to resistance, a feature necessary to avoid any possible interference with our innate immune system.

Original languageEnglish
Article number4538
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - dec. 1 2019

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peptides
Peptides
Bacteria
bacteria
antibiotics
Anti-Bacterial Agents
Gene Amplification
Immune system
Point Mutation
immune systems
Escherichia coli
plasmids
Immune System
Plasmids
Soil
mutations
Escherichia
genes
Soils
soils

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Integrated evolutionary analysis reveals antimicrobial peptides with limited resistance. / Spohn, Réka; Daruka, Lejla; Lázár, Viktória; Martins, Ana; Vidovics, Fanni; Grézal, Gábor; Méhi, Orsolya; Kintses, Bálint; Számel, Mónika; Jangir, Pramod K.; Csörgő, Bálint; Györkei, Ádám; Bódi, Zoltán; Faragó, Anikó; Bodai, L.; Földesi, I.; Kata, Diána; Maróti, Gergely; Pap, Bernadett; Wirth, Roland; Papp, B.; Pál, C.

In: Nature communications, Vol. 10, No. 1, 4538, 01.12.2019.

Research output: Article

Spohn, R, Daruka, L, Lázár, V, Martins, A, Vidovics, F, Grézal, G, Méhi, O, Kintses, B, Számel, M, Jangir, PK, Csörgő, B, Györkei, Á, Bódi, Z, Faragó, A, Bodai, L, Földesi, I, Kata, D, Maróti, G, Pap, B, Wirth, R, Papp, B & Pál, C 2019, 'Integrated evolutionary analysis reveals antimicrobial peptides with limited resistance', Nature communications, vol. 10, no. 1, 4538. https://doi.org/10.1038/s41467-019-12364-6
Spohn, Réka ; Daruka, Lejla ; Lázár, Viktória ; Martins, Ana ; Vidovics, Fanni ; Grézal, Gábor ; Méhi, Orsolya ; Kintses, Bálint ; Számel, Mónika ; Jangir, Pramod K. ; Csörgő, Bálint ; Györkei, Ádám ; Bódi, Zoltán ; Faragó, Anikó ; Bodai, L. ; Földesi, I. ; Kata, Diána ; Maróti, Gergely ; Pap, Bernadett ; Wirth, Roland ; Papp, B. ; Pál, C. / Integrated evolutionary analysis reveals antimicrobial peptides with limited resistance. In: Nature communications. 2019 ; Vol. 10, No. 1.
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