Inhibition of tumor induction in tobacco by Agrobacterium tumefaciens and nodulation induced by Rhizobium meliloti in the presence of phenothiazines and structurally related compounds

M. Szabo, J. Molnár, Z. Bánfalvi, N. Motohashi

Research output: Article

4 Citations (Scopus)

Abstract

Plasmids of Agrobacterium tumefaciens and Rhizobium meliloti carrying Kanamycin resistance genes were eliminated from 1.4 to 0.2% of the growing bacterial cultures by promethazine and imipramine. As a result of plasmid elimination, the A. tumefaciens plasmidless isolate was not able to induce crown gall tumor on tobacco plants. The plasmidless R. meliloti strain failed to induce nodule formation on alfalfa plants. The efficiency of nodulation was decreased when the bacteria were grown in the presence of the drugs. The antiplasmid effects of the drugs were not prevented by opines, (nopaline and octopine) in Escherichia coli Flac cells.

Original languageEnglish
Pages (from-to)1667-1670
Number of pages4
JournalAnticancer Research
Volume12
Issue number5
Publication statusPublished - 1992

Fingerprint

Sinorhizobium meliloti
Phenothiazines
Agrobacterium tumefaciens
Tobacco
Plasmids
Plant Tumors
Kanamycin Resistance
Promethazine
Medicago sativa
Imipramine
Pharmaceutical Preparations
Neoplasms
Escherichia coli
Bacteria
Genes
nopaline
octopine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Inhibition of tumor induction in tobacco by Agrobacterium tumefaciens and nodulation induced by Rhizobium meliloti in the presence of phenothiazines and structurally related compounds",
abstract = "Plasmids of Agrobacterium tumefaciens and Rhizobium meliloti carrying Kanamycin resistance genes were eliminated from 1.4 to 0.2{\%} of the growing bacterial cultures by promethazine and imipramine. As a result of plasmid elimination, the A. tumefaciens plasmidless isolate was not able to induce crown gall tumor on tobacco plants. The plasmidless R. meliloti strain failed to induce nodule formation on alfalfa plants. The efficiency of nodulation was decreased when the bacteria were grown in the presence of the drugs. The antiplasmid effects of the drugs were not prevented by opines, (nopaline and octopine) in Escherichia coli Flac cells.",
keywords = "Chlorpromazine, Imipramin, Nodulation, Plasmid elimination, Tumor formation",
author = "M. Szabo and J. Moln{\'a}r and Z. B{\'a}nfalvi and N. Motohashi",
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language = "English",
volume = "12",
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journal = "Anticancer Research",
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T1 - Inhibition of tumor induction in tobacco by Agrobacterium tumefaciens and nodulation induced by Rhizobium meliloti in the presence of phenothiazines and structurally related compounds

AU - Szabo, M.

AU - Molnár, J.

AU - Bánfalvi, Z.

AU - Motohashi, N.

PY - 1992

Y1 - 1992

N2 - Plasmids of Agrobacterium tumefaciens and Rhizobium meliloti carrying Kanamycin resistance genes were eliminated from 1.4 to 0.2% of the growing bacterial cultures by promethazine and imipramine. As a result of plasmid elimination, the A. tumefaciens plasmidless isolate was not able to induce crown gall tumor on tobacco plants. The plasmidless R. meliloti strain failed to induce nodule formation on alfalfa plants. The efficiency of nodulation was decreased when the bacteria were grown in the presence of the drugs. The antiplasmid effects of the drugs were not prevented by opines, (nopaline and octopine) in Escherichia coli Flac cells.

AB - Plasmids of Agrobacterium tumefaciens and Rhizobium meliloti carrying Kanamycin resistance genes were eliminated from 1.4 to 0.2% of the growing bacterial cultures by promethazine and imipramine. As a result of plasmid elimination, the A. tumefaciens plasmidless isolate was not able to induce crown gall tumor on tobacco plants. The plasmidless R. meliloti strain failed to induce nodule formation on alfalfa plants. The efficiency of nodulation was decreased when the bacteria were grown in the presence of the drugs. The antiplasmid effects of the drugs were not prevented by opines, (nopaline and octopine) in Escherichia coli Flac cells.

KW - Chlorpromazine

KW - Imipramin

KW - Nodulation

KW - Plasmid elimination

KW - Tumor formation

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