Based on cDNA sequence data ε chain-specific antisense oligonucleotides were synthesized and checked on in vitro IgE production. Using peripheral blood cells from a hypereosinophilic patient and a human IgE myeloma cell line, U266, marked reduction of in vitro IgE production measured by PRIST was observed. The effect of ε antisenses proved to be isotype specific since IgG production by both peripheral blood cells and a lymphoma cell line, CESS, was not affected. Moreover, the expression of other markers on U266 (interleukin- 6 receptor and gp 130) were not influenced by ε-specific antisense oligonucleotides.
ASJC Scopus subject areas
- Immunology and Allergy