Influence of water content of the mobile phase on chromatographic performance in adsorption chromatography

L. Szepesy, C. Combellas, M. Caude, R. Rosset

Research output: Article

12 Citations (Scopus)

Abstract

Silicas (LiChrosorb Si 60 and Si 100) and aluminas (Alox T, Woelm, A5Y Spherisorb) with different specific surface areas were investigated with chloroform and dichloroethane as mobile phases containing various amounts of water in the range 0-600 ppm. The water content of the eluent was adjusted by adding a measured amount of water and was determined by the Karl Fisher method. Measurements were carried out with different mobile phase velocities and reduced plate heights were determined. From the measured data, the capacity ratio (k′), selectivity (α), number of theoretical plates (Nmax) and resolution (RS) were determined and plotted as a function of the water content of the mobile phase. It was established that in order to evaluate the influence of the water content of the mobile phase on the chromatographic performance of an adsorbent packing, not only changes in the capacity ratios but also the changes in the selectivities and efficiencies and from these data the changes in resolution should be determined.

Original languageEnglish
Pages (from-to)65-78
Number of pages14
JournalJournal of Chromatography A
Volume237
Issue number1
DOIs
Publication statusPublished - márc. 5 1982

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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