Induction of ethylene synthesis and lipid peroxidation in damaged or TMV-infected tobacco leaf tissues by light

B. Barna, M. Pogány, J. Koehl, I. Heiser, E. F. Elstner

Research output: Article

6 Citations (Scopus)

Abstract

The effect of light on ethylene and ethane production in damaged leaf tissues was investigated. When whole leaves of tobacco cv. Samsun NN were damaged with liquid nitrogen, the ethylene formation was the highest, if 100 % of leaves were injured and were kept in the light, the lowest when leaves after 100 % injury were kept in darkness. Ethane production (lipid peroxidation) could be detected only in damaged, but not in control leaves, and was much higher in light than in darkness. In addition, there was a strong degradation of chlorophyll of damaged leaves kept in light. In light aminoethoxy-vinylglycine (AVG) inhibited ethylene formation in control, non-damaged whole leaves effectively, but in leaves with 100 % damage the inhibitory effect was much weaker and similar to the effect of propyl gallate (PG), a free radical scavenger. Both AVG and PG treatments decreased ethylene formation by control leaf discs and discs with 100 % damage. Ethane production was significantly inhibited by PG and slightly by AVG in the case of 100 % damage. Tiron, another free radical scavenger gave similar results on leaf discs as PG did. Paraquat (methylviologen, Pq), as a photosynthesis inhibiting and reactive oxygen species (ROS) producing herbicide produced a large amount of ethylene and ethane in light but very small amount in darkness. In accordance, tobacco mosaic virus (TMV) infection on the necrotic host resulted in significantly larger amount of ethylene and ethane formation in light than in darkness. We conclude that ethylene and ethane production of damaged plant tissues is strongly induced by light and ROS that are involved in this induction.

Original languageEnglish
Pages (from-to)1905-1914
Number of pages10
JournalActa Physiologiae Plantarum
Volume34
Issue number5
DOIs
Publication statusPublished - szept. 2012

Fingerprint

Tobacco Mosaic Virus
Tobacco mosaic virus
Lipid Peroxidation
Ethane
Tobacco
ethylene
lipid peroxidation
tobacco
ethane
Propyl Gallate
Light
synthesis
Darkness
propyl gallate
leaves
Free Radical Scavengers
ethylene production
free radical scavengers
Reactive Oxygen Species
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Induction of ethylene synthesis and lipid peroxidation in damaged or TMV-infected tobacco leaf tissues by light. / Barna, B.; Pogány, M.; Koehl, J.; Heiser, I.; Elstner, E. F.

In: Acta Physiologiae Plantarum, Vol. 34, No. 5, 09.2012, p. 1905-1914.

Research output: Article

Barna, B. ; Pogány, M. ; Koehl, J. ; Heiser, I. ; Elstner, E. F. / Induction of ethylene synthesis and lipid peroxidation in damaged or TMV-infected tobacco leaf tissues by light. In: Acta Physiologiae Plantarum. 2012 ; Vol. 34, No. 5. pp. 1905-1914.
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abstract = "The effect of light on ethylene and ethane production in damaged leaf tissues was investigated. When whole leaves of tobacco cv. Samsun NN were damaged with liquid nitrogen, the ethylene formation was the highest, if 100 {\%} of leaves were injured and were kept in the light, the lowest when leaves after 100 {\%} injury were kept in darkness. Ethane production (lipid peroxidation) could be detected only in damaged, but not in control leaves, and was much higher in light than in darkness. In addition, there was a strong degradation of chlorophyll of damaged leaves kept in light. In light aminoethoxy-vinylglycine (AVG) inhibited ethylene formation in control, non-damaged whole leaves effectively, but in leaves with 100 {\%} damage the inhibitory effect was much weaker and similar to the effect of propyl gallate (PG), a free radical scavenger. Both AVG and PG treatments decreased ethylene formation by control leaf discs and discs with 100 {\%} damage. Ethane production was significantly inhibited by PG and slightly by AVG in the case of 100 {\%} damage. Tiron, another free radical scavenger gave similar results on leaf discs as PG did. Paraquat (methylviologen, Pq), as a photosynthesis inhibiting and reactive oxygen species (ROS) producing herbicide produced a large amount of ethylene and ethane in light but very small amount in darkness. In accordance, tobacco mosaic virus (TMV) infection on the necrotic host resulted in significantly larger amount of ethylene and ethane formation in light than in darkness. We conclude that ethylene and ethane production of damaged plant tissues is strongly induced by light and ROS that are involved in this induction.",
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N2 - The effect of light on ethylene and ethane production in damaged leaf tissues was investigated. When whole leaves of tobacco cv. Samsun NN were damaged with liquid nitrogen, the ethylene formation was the highest, if 100 % of leaves were injured and were kept in the light, the lowest when leaves after 100 % injury were kept in darkness. Ethane production (lipid peroxidation) could be detected only in damaged, but not in control leaves, and was much higher in light than in darkness. In addition, there was a strong degradation of chlorophyll of damaged leaves kept in light. In light aminoethoxy-vinylglycine (AVG) inhibited ethylene formation in control, non-damaged whole leaves effectively, but in leaves with 100 % damage the inhibitory effect was much weaker and similar to the effect of propyl gallate (PG), a free radical scavenger. Both AVG and PG treatments decreased ethylene formation by control leaf discs and discs with 100 % damage. Ethane production was significantly inhibited by PG and slightly by AVG in the case of 100 % damage. Tiron, another free radical scavenger gave similar results on leaf discs as PG did. Paraquat (methylviologen, Pq), as a photosynthesis inhibiting and reactive oxygen species (ROS) producing herbicide produced a large amount of ethylene and ethane in light but very small amount in darkness. In accordance, tobacco mosaic virus (TMV) infection on the necrotic host resulted in significantly larger amount of ethylene and ethane formation in light than in darkness. We conclude that ethylene and ethane production of damaged plant tissues is strongly induced by light and ROS that are involved in this induction.

AB - The effect of light on ethylene and ethane production in damaged leaf tissues was investigated. When whole leaves of tobacco cv. Samsun NN were damaged with liquid nitrogen, the ethylene formation was the highest, if 100 % of leaves were injured and were kept in the light, the lowest when leaves after 100 % injury were kept in darkness. Ethane production (lipid peroxidation) could be detected only in damaged, but not in control leaves, and was much higher in light than in darkness. In addition, there was a strong degradation of chlorophyll of damaged leaves kept in light. In light aminoethoxy-vinylglycine (AVG) inhibited ethylene formation in control, non-damaged whole leaves effectively, but in leaves with 100 % damage the inhibitory effect was much weaker and similar to the effect of propyl gallate (PG), a free radical scavenger. Both AVG and PG treatments decreased ethylene formation by control leaf discs and discs with 100 % damage. Ethane production was significantly inhibited by PG and slightly by AVG in the case of 100 % damage. Tiron, another free radical scavenger gave similar results on leaf discs as PG did. Paraquat (methylviologen, Pq), as a photosynthesis inhibiting and reactive oxygen species (ROS) producing herbicide produced a large amount of ethylene and ethane in light but very small amount in darkness. In accordance, tobacco mosaic virus (TMV) infection on the necrotic host resulted in significantly larger amount of ethylene and ethane formation in light than in darkness. We conclude that ethylene and ethane production of damaged plant tissues is strongly induced by light and ROS that are involved in this induction.

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