Increased Cd2+ biosorption capability of Aspergillus nidulans elicited by crpA deletion

Imre Boczonádi, Zsófia Török, Ágnes Jakab, Gábor Kónya, Klaudia Gyurcsó, Edina Baranyai, Zoltán Szoboszlai, Boglárka Döncző, István Fábián, Éva Leiter, Mi Kyung Lee, László Csernoch, Jae Hyuk Yu, Zsófia Kertész, Tamás Emri, István Pócsi

Research output: Article

Abstract

The P-type ATPase CrpA is an important Cu2+/Cd2+ pump in the Aspergilli, significantly contributing to the heavy metal stress tolerance of these ascomycetous fungi. As expected, the deletion of crpA resulted in Cu2+/Cd2+-sensitive phenotypes in Aspergillus nidulans on stress agar plates inoculated with conidia. Nevertheless, paradoxical growth stimulations were observed with the ΔcrpA strain in both standard Cu2+ stress agar plate experiments and cellophane colony harvest (CCH) cultures, when exposed to Cd2+. These observations reflect efficient compensatory mechanisms for the loss of CrpA operating under these experimental conditions. It is remarkable that the ΔcrpA strain showed a 2.7 times higher Cd biosorption capacity in CCH cultures, which may facilitate the development of new, fungal biomass-based bioremediation technologies to extract harmful Cd2+ ions from the environment. The nullification of crpA also significantly changed the spatial distribution of Cu and Cd in CCH cultures, as demonstrated by the combined particle-induced X-ray emission and scanning transmission ion microscopy technique. Most important, the centers of gravity for Cu and Cd accumulations of the ΔcrpA colonies shifted toward the older regions as compared with wild-type surface cultures.

Original languageEnglish
Pages (from-to)574-584
Number of pages11
JournalJournal of basic microbiology
Volume60
Issue number7
DOIs
Publication statusPublished - júl. 1 2020

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Increased Cd<sup>2+</sup> biosorption capability of Aspergillus nidulans elicited by crpA deletion'. Together they form a unique fingerprint.

  • Cite this

    Boczonádi, I., Török, Z., Jakab, Á., Kónya, G., Gyurcsó, K., Baranyai, E., Szoboszlai, Z., Döncző, B., Fábián, I., Leiter, É., Lee, M. K., Csernoch, L., Yu, J. H., Kertész, Z., Emri, T., & Pócsi, I. (2020). Increased Cd2+ biosorption capability of Aspergillus nidulans elicited by crpA deletion. Journal of basic microbiology, 60(7), 574-584. https://doi.org/10.1002/jobm.202000112