Increase in the Relative Fitness of a Plant Virus RNA Associated with Its Recombinant Nature

Beatriz Fernández-Cuartero, J. Burgyán, Miguel A. Aranda, K. Salánki, Enrique Moriones, Fernando García-Arenal

Research output: Article

77 Citations (Scopus)

Abstract

A recombination event at the 3′ end of RNA 3 in a pseudorecombinant virus (C1C2T3) having RNAs 1 and 2 from TrK7-cucumber mosaic virus (CMV) and RNA 3 from P-tomato aspermy virus (TAV) was detected by ribonuclease protection assay (RPA). Sequence analysis of the 3′ end of RNA 3 of C1C2T3 and of its parental CMV and TAV strains showed that RNA 3 of C1C2T3 had a hybrid nature in which most of the 3′ noncoding region (3′ ncr), including the whole 3′ terminal tRNA-like structure, was derived from CMV RNA 2 by recombination. Recombination may have occurred in two steps. The first one would have caused the duplication of a large region 5′ to the 3′ end tRNA-like structure, derived from TAV and from CMV. The second one would have eliminated the TAV-derived sequences in this duplicated region. Competition experiments in tobacco plants showed that in a context of RNA 1 + 2 from CMV, RNA 3 from TAV is outcompeted by RNA 3 of CMV, but the recombinant TAV RNA 3 with the 3′ end of CMV outcompetes both TAV and CMV RNA 3. This shows an increase in relative fitness associated with the recombinant nature of RNA 3. Our results document the potential importance of RNA-RNA recombination in the determination of the genetic structure of RNA viral populations.

Original languageEnglish
Pages (from-to)373-377
Number of pages5
JournalVirology
Volume203
Issue number2
DOIs
Publication statusPublished - szept. 1994

Fingerprint

Plant RNA
Plant Viruses
Cucumovirus
RNA
Lycopersicon esculentum
Viruses
Genetic Recombination
Transfer RNA
Genetic Structures
RNA Viruses
Viral RNA
Ribonucleases
Tobacco

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Increase in the Relative Fitness of a Plant Virus RNA Associated with Its Recombinant Nature. / Fernández-Cuartero, Beatriz; Burgyán, J.; Aranda, Miguel A.; Salánki, K.; Moriones, Enrique; García-Arenal, Fernando.

In: Virology, Vol. 203, No. 2, 09.1994, p. 373-377.

Research output: Article

Fernández-Cuartero, B, Burgyán, J, Aranda, MA, Salánki, K, Moriones, E & García-Arenal, F 1994, 'Increase in the Relative Fitness of a Plant Virus RNA Associated with Its Recombinant Nature', Virology, vol. 203, no. 2, pp. 373-377. https://doi.org/10.1006/viro.1994.1496
Fernández-Cuartero, Beatriz ; Burgyán, J. ; Aranda, Miguel A. ; Salánki, K. ; Moriones, Enrique ; García-Arenal, Fernando. / Increase in the Relative Fitness of a Plant Virus RNA Associated with Its Recombinant Nature. In: Virology. 1994 ; Vol. 203, No. 2. pp. 373-377.
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