In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy

Attila Kolonics, Zsolt Csiszovszki, Eniko R. Toke, Orsolya Lorincz, Dóra Haluszka, R. Szipócs

Research output: Article

9 Citations (Scopus)

Abstract

Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.

Original languageEnglish
Pages (from-to)596-605
Number of pages10
JournalExperimental Dermatology
Volume23
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Nanomedicine
Medical nanotechnology
Langerhans Cells
Confocal Microscopy
Microscopic examination
Scanning
Lasers
Cells
Pathogens
Mannose
Antigen-Presenting Cells
DermaVir
Epidermis
Skin
Animals
Nanoparticles
Genes
Antigens
HIV

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy. / Kolonics, Attila; Csiszovszki, Zsolt; Toke, Eniko R.; Lorincz, Orsolya; Haluszka, Dóra; Szipócs, R.

In: Experimental Dermatology, Vol. 23, No. 8, 2014, p. 596-605.

Research output: Article

Kolonics, Attila ; Csiszovszki, Zsolt ; Toke, Eniko R. ; Lorincz, Orsolya ; Haluszka, Dóra ; Szipócs, R. / In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy. In: Experimental Dermatology. 2014 ; Vol. 23, No. 8. pp. 596-605.
@article{cdf8067466054e8cb0ad95324a88d3e5,
title = "In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy",
abstract = "Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.",
keywords = "EGFP-Langerin knock-in mice, in vivo, Langerhans cells, Multiphoton laser microscopy, Nanomedicine formulation",
author = "Attila Kolonics and Zsolt Csiszovszki and Toke, {Eniko R.} and Orsolya Lorincz and D{\'o}ra Haluszka and R. Szip{\'o}cs",
year = "2014",
doi = "10.1111/exd.12464",
language = "English",
volume = "23",
pages = "596--605",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy

AU - Kolonics, Attila

AU - Csiszovszki, Zsolt

AU - Toke, Eniko R.

AU - Lorincz, Orsolya

AU - Haluszka, Dóra

AU - Szipócs, R.

PY - 2014

Y1 - 2014

N2 - Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.

AB - Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.

KW - EGFP-Langerin knock-in mice

KW - in vivo

KW - Langerhans cells

KW - Multiphoton laser microscopy

KW - Nanomedicine formulation

UR - http://www.scopus.com/inward/record.url?scp=84905391508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905391508&partnerID=8YFLogxK

U2 - 10.1111/exd.12464

DO - 10.1111/exd.12464

M3 - Article

C2 - 24903756

AN - SCOPUS:84905391508

VL - 23

SP - 596

EP - 605

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 8

ER -