In vitro synthesis and identification of lysozyme in human tonsillar cells

Mária Puskás, M. Staub, F. Antoni

Research output: Article

4 Citations (Scopus)

Abstract

Remarkable lysozyme activity was found in human tonsillar cells. The in vitro synthesis of lysozyme has been investigated by [14C]valine incorporation in cell culture. [14C]labeled enzyme has been isolated, and identified as a single band of protein by polyacrylamide electrophoresis. Enzyme synthesis was inhibited by cycloheximide, which caused a 40% inhibition at a concentration of 10 μm, while 1.0 mm blocked the synthesis totally. Separating cells on a discontinuous albumin gradient, a fraction was obtained in which the specific activity of lysozyme was 10 times higher than in unseparated cells. Lysozyme containing cells were identified in agarose-gel plates and appeared as small lymphocytes.

Original languageEnglish
Pages (from-to)359-369
Number of pages11
JournalBiochemical Medicine
Volume13
Issue number4
DOIs
Publication statusPublished - 1975

Fingerprint

Muramidase
Lymphocytes
Valine
Enzymes
Cycloheximide
Electrophoresis
Cell culture
Sepharose
Albumins
Cell Culture Techniques
Gels
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

In vitro synthesis and identification of lysozyme in human tonsillar cells. / Puskás, Mária; Staub, M.; Antoni, F.

In: Biochemical Medicine, Vol. 13, No. 4, 1975, p. 359-369.

Research output: Article

Puskás, Mária ; Staub, M. ; Antoni, F. / In vitro synthesis and identification of lysozyme in human tonsillar cells. In: Biochemical Medicine. 1975 ; Vol. 13, No. 4. pp. 359-369.
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