In vitro neutralization of T-2 toxin toxicity by a monoclonal antibody

F. Minervini, A. Gyongyosi-Horvath, G. Lucivero, A. Visconti, I. Barna-Vetró, L. Solti

Research output: Article

2 Citations (Scopus)

Abstract

A T-2 toxin specific monoclonal antibody, IgG1 K, with a low level of ELISA cross-reactivity to Acetyl T-2, HT-2, and also T-2 toxins has been produced. The ability of this monoclonal antibody to neutralize the cytotoxicity of T-2 toxin in PHA stimulated cultures of human lymphocytes was determined by the MTT method. The complete neutralization of the toxic effect of 0.02 μM T-2 toxin was obtained with 0.03 μM of MoAb, whereas the 50% neutralizing dose (ND50) was observed at 0.009 μM of MoAb. Partial neutralization was observed with Acetyl T-2 toxin (ND50 = 0.038 μM) and HT-2 (ND50 = 0.94 μM). These results could represent a rational for clinical use of T-2 toxin specific monoclonal antibody in prophylaxis and therapy of T-2 toxemia.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalNatural Toxins
Volume2
Issue number3
DOIs
Publication statusPublished - 1994

Fingerprint

T-2 Toxin
Toxicity
Monoclonal Antibodies
Toxemia
Lymphocytes
Poisons
Cytotoxicity
Cell culture
In Vitro Techniques
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Toxicology

Cite this

In vitro neutralization of T-2 toxin toxicity by a monoclonal antibody. / Minervini, F.; Gyongyosi-Horvath, A.; Lucivero, G.; Visconti, A.; Barna-Vetró, I.; Solti, L.

In: Natural Toxins, Vol. 2, No. 3, 1994, p. 111-114.

Research output: Article

Minervini, F, Gyongyosi-Horvath, A, Lucivero, G, Visconti, A, Barna-Vetró, I & Solti, L 1994, 'In vitro neutralization of T-2 toxin toxicity by a monoclonal antibody', Natural Toxins, vol. 2, no. 3, pp. 111-114. https://doi.org/10.1002/nt.2620020304
Minervini, F. ; Gyongyosi-Horvath, A. ; Lucivero, G. ; Visconti, A. ; Barna-Vetró, I. ; Solti, L. / In vitro neutralization of T-2 toxin toxicity by a monoclonal antibody. In: Natural Toxins. 1994 ; Vol. 2, No. 3. pp. 111-114.
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