In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use

Hans C. Helms, N. Joan Abbott, Malgorzata Burek, Romeo Cecchelli, Pierre Olivier Couraud, Maria A. Deli, Carola Förster, Hans J. Galla, Ignacio A. Romero, Eric V. Shusta, Matthew J. Stebbins, Elodie Vandenhaute, Babette Weksler, Birger Brodin

Research output: Review article

200 Citations (Scopus)

Abstract

The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This â €œ blood-brain barrierâ € function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. As an ideal cell culture model of the blood-brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described.

Original languageEnglish
Pages (from-to)862-890
Number of pages29
JournalJournal of Cerebral Blood Flow and Metabolism
Volume36
Issue number5
DOIs
Publication statusPublished - jan. 1 2015

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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    Helms, H. C., Abbott, N. J., Burek, M., Cecchelli, R., Couraud, P. O., Deli, M. A., Förster, C., Galla, H. J., Romero, I. A., Shusta, E. V., Stebbins, M. J., Vandenhaute, E., Weksler, B., & Brodin, B. (2015). In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use. Journal of Cerebral Blood Flow and Metabolism, 36(5), 862-890. https://doi.org/10.1177/0271678X16630991