In vitro anti-diabetic activity and chemical characterization of an apolar fraction of morus alba leaf water extract

Attila Hunyadi, Katalin Veres, Balazs Danko, Zoltan Kele, Edit Weber, Anasztazia Hetenyi, Istvan Zupko, Tusty Jiuan Hsieh

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19 Citations (Scopus)


The tea from the white mulberry (Morus alba L.) leaf is a worldwide known traditional medicine of type II diabetes. Here, we report the investigation of the dichloromethane-soluble fraction obtained in a 0.24% m/m yield from the hot water extract of mulberry leaves. A significant, dose-dependent activity was found by means of the 24-h glucose consumption of fully differentiated adipocytes both in the absence and presence of insulin. The fraction was characterized by HPLC-DAD, GC-MS and GC-FID. The main constituent (40.3% by means of GC-FID) was isolated and identified as loliolide by EIMS, HRESIMS and NMR spectroscopy. In the active fraction benzyl alcohol, ethyl benzoate, t-cinnamic acid, p-hydroxyacetophenone, t-coniferyl alcohol and synapil alcohol were also identified by GC-MS and quantified by GC-FID (0.7, 1.3, 1.5, 2.9, 7.5 and 2.6%, respectively).

Original languageEnglish
Pages (from-to)847-851
Number of pages5
JournalPhytotherapy Research
Issue number6
Publication statusPublished - jún. 1 2013


ASJC Scopus subject areas

  • Pharmacology

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