In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method

Olga Bolshakova, A. Borisenkova, Marina Suyasova, Victor Sedov, Aleksandra Slobodina, Svetlana Timoshenko, E. Varfolomeeva, Ilia Golomidov, Vasily Lebedev, V. Aksenov, Svetlana Sarantseva

Research output: Article

Abstract

The toxicity of C60(OH)30, C70(OH)30, and C120O(OH)n fullerenols, prepared by a new original method, has been studied. This method allowed us to obtain high-purity fullerenols and eliminate the risks of synthesis of preparations containing insoluble fractions contaminated with impurities such as fullerenes not completely reacted by hydroxylation. All fullerenols were detected inside cultured cells. The MTT assay as well as the analysis of apoptosis and cell cycle showed that С60(ОН)30 and С70(OH)30 are non-toxic for cultured V79 и HeLa cells at concentrations exceeding physiological levels by an order of magnitude. С120O(OH)n caused low toxicity. Studies in Drosophila melanogaster showed that any preparations used did not result in a decreased lifespan or in behavior abnormalities in flies.

Original languageEnglish
Article number109945
JournalMaterials Science and Engineering C
Volume104
DOIs
Publication statusPublished - nov. 1 2019

Fingerprint

toxicity
Toxicity
Cells
Drosophila
Hydroxylation
preparation
abnormalities
apoptosis
Cell death
Fullerenes
cultured cells
fullerenes
Assays
purity
Impurities
impurities
cycles
synthesis
cells
Apoptosis

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Bolshakova, O., Borisenkova, A., Suyasova, M., Sedov, V., Slobodina, A., Timoshenko, S., ... Sarantseva, S. (2019). In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method. Materials Science and Engineering C, 104, [109945]. https://doi.org/10.1016/j.msec.2019.109945

In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method. / Bolshakova, Olga; Borisenkova, A.; Suyasova, Marina; Sedov, Victor; Slobodina, Aleksandra; Timoshenko, Svetlana; Varfolomeeva, E.; Golomidov, Ilia; Lebedev, Vasily; Aksenov, V.; Sarantseva, Svetlana.

In: Materials Science and Engineering C, Vol. 104, 109945, 01.11.2019.

Research output: Article

Bolshakova, O, Borisenkova, A, Suyasova, M, Sedov, V, Slobodina, A, Timoshenko, S, Varfolomeeva, E, Golomidov, I, Lebedev, V, Aksenov, V & Sarantseva, S 2019, 'In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method', Materials Science and Engineering C, vol. 104, 109945. https://doi.org/10.1016/j.msec.2019.109945
Bolshakova, Olga ; Borisenkova, A. ; Suyasova, Marina ; Sedov, Victor ; Slobodina, Aleksandra ; Timoshenko, Svetlana ; Varfolomeeva, E. ; Golomidov, Ilia ; Lebedev, Vasily ; Aksenov, V. ; Sarantseva, Svetlana. / In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method. In: Materials Science and Engineering C. 2019 ; Vol. 104.
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AU - Timoshenko, Svetlana

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