Immunolocalization of electroneutral Na+-HCO3 - cotransporters in human and rat salivary glands

V. Gresz, T. H. Kwon, H. Vorum, T. Zelles, I. Kurtz, M. C. Steward, C. Aalkjaer, S. Nielsen

Research output: Article

26 Citations (Scopus)

Abstract

Patterns of salivary HCO3 secretion vary widely among species and among individual glands. In particular, virtually nothing is known about the molecular identity of the HCO3 - transporters involved in human salivary secretion. We have therefore examined the distribution of several known members of the Na+-HCO3 - cotransporter (NBC) family in the parotid and submandibular glands. By use of a combination of RT-PCR and immunoblotting analyses, the electroneutral cotransporters NBC3 and NBCn1 mRNA and protein expression were detected in both human and rat tissues. Immunohistochemistry demonstrated that NBC3 was present at the apical membranes of acinar and duct cells in both human and rat parotid and submandibular glands. NBCn1 was strongly expressed at the basolateral membrane of striated duct cells but not in the acinar cells in the human salivary glands, whereas little or no NBCn1 labeling was observed in the rat salivary glands. The presence of NBCn1 at the basolateral membrane of human striated duct cells suggests that it may contribute to ductal HCO3 - secretion. In contrast, the expression of NBC3 at the apical membranes of acinar and duct cells in both human and rat salivary glands indicates a possible role of this isoform in HCO3 - salvage under resting conditions.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number2 46-2
Publication statusPublished - 2002

Fingerprint

Salivary Glands
Acinar Cells
Membranes
Submandibular Gland
Parotid Gland
Immunoblotting
Protein Isoforms
Immunohistochemistry
Polymerase Chain Reaction
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Immunolocalization of electroneutral Na+-HCO3 - cotransporters in human and rat salivary glands. / Gresz, V.; Kwon, T. H.; Vorum, H.; Zelles, T.; Kurtz, I.; Steward, M. C.; Aalkjaer, C.; Nielsen, S.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 283, No. 2 46-2, 2002.

Research output: Article

Gresz, V. ; Kwon, T. H. ; Vorum, H. ; Zelles, T. ; Kurtz, I. ; Steward, M. C. ; Aalkjaer, C. ; Nielsen, S. / Immunolocalization of electroneutral Na+-HCO3 - cotransporters in human and rat salivary glands. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 283, No. 2 46-2.
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