Immunogold localisation of ubiquitin-protein conjugates in Sf9 insect cells. Implications for the biogenesis of lysosome-related organelles

Péter Löw, Fergus J. Doherty, Miklos Sass, János Kovács, R. John Mayer, Lajos László

Research output: Article

12 Citations (Scopus)

Abstract

Immunogold electron microscopy with antibodies which primarily detect ubiquitin-protein conjugates shows conjugate-specific gold particles enriched severalfold in acid phosphatase-positive lysosomes and multivesicular bodies in insect Sf9 cells. The observations demonstrate that ubiquitinated proteins are associated with small acid phosphatase-containing primary lysosomes (transport vesicles) and indicate a pathway in which primary lysosomes fuse with multivesicular bodies to generate mature lysosome-related structures.

Original languageEnglish
Pages (from-to)152-156
Number of pages5
JournalFEBS letters
Volume316
Issue number2
DOIs
Publication statusPublished - jan. 25 1993

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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