Immune-mediated skin inflammation is similar in severe atopic dermatitis patients with or without filaggrin mutation

Zsolt Dajnoki, Gabriella Béke, Gábor Mócsai, A. Kapitány, Krisztián Gáspár, Krisztina Hajdu, Gabriella Emri, Bence Nagy, I. Kovács, Lívia Beke, B. Dezső, A. Szegedi

Research output: Article

12 Citations (Scopus)

Abstract

Inflammatory cytokines can impair the skin barrier, but the question as to whether barrier alterations affect keratinocyte immune responses remains unanswered. The aim of this study was to investigate whether immune-mediated skin inflammation differs between severe atopic dermatitis patients with or without filaggrin mutation. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T-cell and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified by immunohistochemistry. The results were confirmed by quantitative PCR analyses. No significant differences were found between the 2 patient groups. Expression of atopic dermatitis-specific cytokines showed significant correlation with histological severity. These findings suggest that the immune-mediated skin inflammation (represented by keratinocyte-derived factors, T-cell and dendritic cell counts) is similar in the 2 patient groups with severe atopic dermatitis, and that immune activation is connected to the severity of the disease rather than to the origin of barrier alterations.

Original languageEnglish
Pages (from-to)645-650
Number of pages6
JournalActa Dermato-Venereologica
Volume96
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Atopic Dermatitis
Inflammation
Skin
Mutation
Cytokines
Keratinocytes
Dendritic Cells
Cell Count
TCF Transcription Factors
CC Chemokines
Immunohistochemistry
filaggrin
Ligands
T-Lymphocytes
Biopsy
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Dermatology

Cite this

Immune-mediated skin inflammation is similar in severe atopic dermatitis patients with or without filaggrin mutation. / Dajnoki, Zsolt; Béke, Gabriella; Mócsai, Gábor; Kapitány, A.; Gáspár, Krisztián; Hajdu, Krisztina; Emri, Gabriella; Nagy, Bence; Kovács, I.; Beke, Lívia; Dezső, B.; Szegedi, A.

In: Acta Dermato-Venereologica, Vol. 96, No. 5, 2016, p. 645-650.

Research output: Article

Dajnoki, Zsolt ; Béke, Gabriella ; Mócsai, Gábor ; Kapitány, A. ; Gáspár, Krisztián ; Hajdu, Krisztina ; Emri, Gabriella ; Nagy, Bence ; Kovács, I. ; Beke, Lívia ; Dezső, B. ; Szegedi, A. / Immune-mediated skin inflammation is similar in severe atopic dermatitis patients with or without filaggrin mutation. In: Acta Dermato-Venereologica. 2016 ; Vol. 96, No. 5. pp. 645-650.
@article{04010279360a49cc9b9ba72f2c2e733e,
title = "Immune-mediated skin inflammation is similar in severe atopic dermatitis patients with or without filaggrin mutation",
abstract = "Inflammatory cytokines can impair the skin barrier, but the question as to whether barrier alterations affect keratinocyte immune responses remains unanswered. The aim of this study was to investigate whether immune-mediated skin inflammation differs between severe atopic dermatitis patients with or without filaggrin mutation. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T-cell and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified by immunohistochemistry. The results were confirmed by quantitative PCR analyses. No significant differences were found between the 2 patient groups. Expression of atopic dermatitis-specific cytokines showed significant correlation with histological severity. These findings suggest that the immune-mediated skin inflammation (represented by keratinocyte-derived factors, T-cell and dendritic cell counts) is similar in the 2 patient groups with severe atopic dermatitis, and that immune activation is connected to the severity of the disease rather than to the origin of barrier alterations.",
keywords = "Atopic dermatitis, Filaggrin, Immunohistochemistry, Innate immunity, Thymic stromal lymphopoietin",
author = "Zsolt Dajnoki and Gabriella B{\'e}ke and G{\'a}bor M{\'o}csai and A. Kapit{\'a}ny and Kriszti{\'a}n G{\'a}sp{\'a}r and Krisztina Hajdu and Gabriella Emri and Bence Nagy and I. Kov{\'a}cs and L{\'i}via Beke and B. Dezső and A. Szegedi",
year = "2016",
doi = "10.2340/00015555-2272",
language = "English",
volume = "96",
pages = "645--650",
journal = "Acta Dermato-Venereologica",
issn = "0001-5555",
publisher = "Society for the Publication of Acta Dermato-Venereologica",
number = "5",

}

TY - JOUR

T1 - Immune-mediated skin inflammation is similar in severe atopic dermatitis patients with or without filaggrin mutation

AU - Dajnoki, Zsolt

AU - Béke, Gabriella

AU - Mócsai, Gábor

AU - Kapitány, A.

AU - Gáspár, Krisztián

AU - Hajdu, Krisztina

AU - Emri, Gabriella

AU - Nagy, Bence

AU - Kovács, I.

AU - Beke, Lívia

AU - Dezső, B.

AU - Szegedi, A.

PY - 2016

Y1 - 2016

N2 - Inflammatory cytokines can impair the skin barrier, but the question as to whether barrier alterations affect keratinocyte immune responses remains unanswered. The aim of this study was to investigate whether immune-mediated skin inflammation differs between severe atopic dermatitis patients with or without filaggrin mutation. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T-cell and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified by immunohistochemistry. The results were confirmed by quantitative PCR analyses. No significant differences were found between the 2 patient groups. Expression of atopic dermatitis-specific cytokines showed significant correlation with histological severity. These findings suggest that the immune-mediated skin inflammation (represented by keratinocyte-derived factors, T-cell and dendritic cell counts) is similar in the 2 patient groups with severe atopic dermatitis, and that immune activation is connected to the severity of the disease rather than to the origin of barrier alterations.

AB - Inflammatory cytokines can impair the skin barrier, but the question as to whether barrier alterations affect keratinocyte immune responses remains unanswered. The aim of this study was to investigate whether immune-mediated skin inflammation differs between severe atopic dermatitis patients with or without filaggrin mutation. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T-cell and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified by immunohistochemistry. The results were confirmed by quantitative PCR analyses. No significant differences were found between the 2 patient groups. Expression of atopic dermatitis-specific cytokines showed significant correlation with histological severity. These findings suggest that the immune-mediated skin inflammation (represented by keratinocyte-derived factors, T-cell and dendritic cell counts) is similar in the 2 patient groups with severe atopic dermatitis, and that immune activation is connected to the severity of the disease rather than to the origin of barrier alterations.

KW - Atopic dermatitis

KW - Filaggrin

KW - Immunohistochemistry

KW - Innate immunity

KW - Thymic stromal lymphopoietin

UR - http://www.scopus.com/inward/record.url?scp=84973635852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973635852&partnerID=8YFLogxK

U2 - 10.2340/00015555-2272

DO - 10.2340/00015555-2272

M3 - Article

C2 - 26536977

AN - SCOPUS:84973635852

VL - 96

SP - 645

EP - 650

JO - Acta Dermato-Venereologica

JF - Acta Dermato-Venereologica

SN - 0001-5555

IS - 5

ER -