IL28B CC genotípus: Védo tényezo és az interferonválasz prediktora krónikus hepatitis C-vírus-infekcióban

A. Pár, G. Pár, István Tornai, F. Szalay, Dalma Várszegi, Edit Fráter, M. Papp, G. Lengyel, J. Fehér, Márta Varga, Judit Gervain, János Schuller, Zsuzsanna Nemes, Zoltán Péterfi, Anna Tusnádi, B. Hunyady, Attila Haragh, Zsolt Szinku, L. Pálinkás, T. BerkiA. Vincze, P. Kisfali, B. Melegh

Research output: Article

4 Citations (Scopus)

Abstract

Introduction: In chronic hepatitis C-virus infection the possible role of gene variants encoding cytokines has become the focus of interest. Aim: The aim of the study was to investigate the effect of IL28B polymorphisms on the outcome of chronic hepatitis C-virus genotype 1 infection in the Hungarian population. In addition, the association between IL28B genotypes and the Th1/Th2 cytokine production of activated peripheral blood monocytes and lymphocytes was evaluated. Method: Total of 748 chronic hepatitis C-virus genotype 1 positive patients (365 males and 383 females, aged between 18 and 82 years; mean age, 54±10 years) were enrolled, of which 420 patients were treated with pegylated interferon plus ribavirin for 24-72 weeks. Of the 420 patients, 195 patients (46.4%) achieved sustained virological response. The IL28B rs12979860 polymorphism was determined using Custom Taqman SNP Genotyping Assays (Applied Biosystems, Life Technologies, Foster, CA, USA). For cytokine studies, tumour necrosis factor-α, interleukin-2, interferon-γ, interleukin-2 and interleukin-4 production by LPS-stimulated monocytes and PMA-ionomycine activated lymphocytes were measured from the supernatant of the cells obtained from 40 hepatitis C-virus infected patients, using FACS-CBA Becton Dickinson test. The cytokine levels were compared in patients with different (CC, CT, TT) IL28B genotypes. Results: The IL28B rs12979860 CC genotype occurred in lower frequency in hepatitis C-virus infected patients than in healthy controls (26.1% vs 51.4%, OR 0.333, p

Original languageHungarian
Pages (from-to)1261-1268
Number of pages8
JournalOrvosi Hetilap
Volume154
Issue number32
DOIs
Publication statusPublished - aug. 1 2013

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Chronic Hepatitis C
Virus Diseases
Hepacivirus
Interferons
Genotype
Cytokines
Therapeutics
Interleukin-2
Monocytes
Lymphocytes
Ribavirin
Protective Factors
Interleukin-4
Single Nucleotide Polymorphism
Tumor Necrosis Factor-alpha
Technology
Infection
Population
Genes

Keywords

  • hepatitis C-vírus
  • hepatitis C-virus
  • IL28B gene polymorphism
  • IL28B-polimorfizmus
  • interferon
  • sustained virological response
  • tartós virológiai válasz
  • Th1/Th2 citokinek
  • Th1/Th2 cytokine production

ASJC Scopus subject areas

  • Medicine(all)

Cite this

IL28B CC genotípus : Védo tényezo és az interferonválasz prediktora krónikus hepatitis C-vírus-infekcióban. / Pár, A.; Pár, G.; Tornai, István; Szalay, F.; Várszegi, Dalma; Fráter, Edit; Papp, M.; Lengyel, G.; Fehér, J.; Varga, Márta; Gervain, Judit; Schuller, János; Nemes, Zsuzsanna; Péterfi, Zoltán; Tusnádi, Anna; Hunyady, B.; Haragh, Attila; Szinku, Zsolt; Pálinkás, L.; Berki, T.; Vincze, A.; Kisfali, P.; Melegh, B.

In: Orvosi Hetilap, Vol. 154, No. 32, 01.08.2013, p. 1261-1268.

Research output: Article

Pár, A. ; Pár, G. ; Tornai, István ; Szalay, F. ; Várszegi, Dalma ; Fráter, Edit ; Papp, M. ; Lengyel, G. ; Fehér, J. ; Varga, Márta ; Gervain, Judit ; Schuller, János ; Nemes, Zsuzsanna ; Péterfi, Zoltán ; Tusnádi, Anna ; Hunyady, B. ; Haragh, Attila ; Szinku, Zsolt ; Pálinkás, L. ; Berki, T. ; Vincze, A. ; Kisfali, P. ; Melegh, B. / IL28B CC genotípus : Védo tényezo és az interferonválasz prediktora krónikus hepatitis C-vírus-infekcióban. In: Orvosi Hetilap. 2013 ; Vol. 154, No. 32. pp. 1261-1268.
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abstract = "Introduction: In chronic hepatitis C-virus infection the possible role of gene variants encoding cytokines has become the focus of interest. Aim: The aim of the study was to investigate the effect of IL28B polymorphisms on the outcome of chronic hepatitis C-virus genotype 1 infection in the Hungarian population. In addition, the association between IL28B genotypes and the Th1/Th2 cytokine production of activated peripheral blood monocytes and lymphocytes was evaluated. Method: Total of 748 chronic hepatitis C-virus genotype 1 positive patients (365 males and 383 females, aged between 18 and 82 years; mean age, 54±10 years) were enrolled, of which 420 patients were treated with pegylated interferon plus ribavirin for 24-72 weeks. Of the 420 patients, 195 patients (46.4{\%}) achieved sustained virological response. The IL28B rs12979860 polymorphism was determined using Custom Taqman SNP Genotyping Assays (Applied Biosystems, Life Technologies, Foster, CA, USA). For cytokine studies, tumour necrosis factor-α, interleukin-2, interferon-γ, interleukin-2 and interleukin-4 production by LPS-stimulated monocytes and PMA-ionomycine activated lymphocytes were measured from the supernatant of the cells obtained from 40 hepatitis C-virus infected patients, using FACS-CBA Becton Dickinson test. The cytokine levels were compared in patients with different (CC, CT, TT) IL28B genotypes. Results: The IL28B rs12979860 CC genotype occurred in lower frequency in hepatitis C-virus infected patients than in healthy controls (26.1{\%} vs 51.4{\%}, OR 0.333, p",
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TY - JOUR

T1 - IL28B CC genotípus

T2 - Védo tényezo és az interferonválasz prediktora krónikus hepatitis C-vírus-infekcióban

AU - Pár, A.

AU - Pár, G.

AU - Tornai, István

AU - Szalay, F.

AU - Várszegi, Dalma

AU - Fráter, Edit

AU - Papp, M.

AU - Lengyel, G.

AU - Fehér, J.

AU - Varga, Márta

AU - Gervain, Judit

AU - Schuller, János

AU - Nemes, Zsuzsanna

AU - Péterfi, Zoltán

AU - Tusnádi, Anna

AU - Hunyady, B.

AU - Haragh, Attila

AU - Szinku, Zsolt

AU - Pálinkás, L.

AU - Berki, T.

AU - Vincze, A.

AU - Kisfali, P.

AU - Melegh, B.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Introduction: In chronic hepatitis C-virus infection the possible role of gene variants encoding cytokines has become the focus of interest. Aim: The aim of the study was to investigate the effect of IL28B polymorphisms on the outcome of chronic hepatitis C-virus genotype 1 infection in the Hungarian population. In addition, the association between IL28B genotypes and the Th1/Th2 cytokine production of activated peripheral blood monocytes and lymphocytes was evaluated. Method: Total of 748 chronic hepatitis C-virus genotype 1 positive patients (365 males and 383 females, aged between 18 and 82 years; mean age, 54±10 years) were enrolled, of which 420 patients were treated with pegylated interferon plus ribavirin for 24-72 weeks. Of the 420 patients, 195 patients (46.4%) achieved sustained virological response. The IL28B rs12979860 polymorphism was determined using Custom Taqman SNP Genotyping Assays (Applied Biosystems, Life Technologies, Foster, CA, USA). For cytokine studies, tumour necrosis factor-α, interleukin-2, interferon-γ, interleukin-2 and interleukin-4 production by LPS-stimulated monocytes and PMA-ionomycine activated lymphocytes were measured from the supernatant of the cells obtained from 40 hepatitis C-virus infected patients, using FACS-CBA Becton Dickinson test. The cytokine levels were compared in patients with different (CC, CT, TT) IL28B genotypes. Results: The IL28B rs12979860 CC genotype occurred in lower frequency in hepatitis C-virus infected patients than in healthy controls (26.1% vs 51.4%, OR 0.333, p

AB - Introduction: In chronic hepatitis C-virus infection the possible role of gene variants encoding cytokines has become the focus of interest. Aim: The aim of the study was to investigate the effect of IL28B polymorphisms on the outcome of chronic hepatitis C-virus genotype 1 infection in the Hungarian population. In addition, the association between IL28B genotypes and the Th1/Th2 cytokine production of activated peripheral blood monocytes and lymphocytes was evaluated. Method: Total of 748 chronic hepatitis C-virus genotype 1 positive patients (365 males and 383 females, aged between 18 and 82 years; mean age, 54±10 years) were enrolled, of which 420 patients were treated with pegylated interferon plus ribavirin for 24-72 weeks. Of the 420 patients, 195 patients (46.4%) achieved sustained virological response. The IL28B rs12979860 polymorphism was determined using Custom Taqman SNP Genotyping Assays (Applied Biosystems, Life Technologies, Foster, CA, USA). For cytokine studies, tumour necrosis factor-α, interleukin-2, interferon-γ, interleukin-2 and interleukin-4 production by LPS-stimulated monocytes and PMA-ionomycine activated lymphocytes were measured from the supernatant of the cells obtained from 40 hepatitis C-virus infected patients, using FACS-CBA Becton Dickinson test. The cytokine levels were compared in patients with different (CC, CT, TT) IL28B genotypes. Results: The IL28B rs12979860 CC genotype occurred in lower frequency in hepatitis C-virus infected patients than in healthy controls (26.1% vs 51.4%, OR 0.333, p

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KW - hepatitis C-virus

KW - IL28B gene polymorphism

KW - IL28B-polimorfizmus

KW - interferon

KW - sustained virological response

KW - tartós virológiai válasz

KW - Th1/Th2 citokinek

KW - Th1/Th2 cytokine production

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