IL28B and IL10R-1087 polymorphisms are protective for chronic genotype 1 HCV infection and predictors of response to interferon-based therapy in an East-Central European cohort

A. Pár, G. Pár, István Tornai, F. Szalay, Dalma Várszegi, Edit Fráter, M. Papp, G. Lengyel, J. Fehér, Márta Varga, Judit Gervain, János Schuller, Zsuzsanna Nemes, Zoltán Péterfi, Anna Tusnádi, B. Hunyady, Attila Haragh, Zsolt Szinku, A. Vincze, L. SzeredayP. Kisfali, B. Melegh

Research output: Article

10 Citations (Scopus)

Abstract

Background: Previous studies have shown that single nucleotide polymorphisms (SNP) in IL28B and IL10R are associated with sustained virological response (SVR) in chronic hepatitis C patients treated with pegilated interferon plus ribavirin (P/R). The present study extends our earlier investigations on a large East-Central European cohort. The allele frequencies of IL28B and IL10R in genotype 1 HCV infection were compared with that of healthy controls for the purpose of examining the relationship between the polymorphisms and the SVR to P/R treatment. Methods: A total of 748 chronic HCV1 infected patients (365 male, 383 female; 18-82 years) and 105 voluntary blood donors as controls were enrolled. Four hundred and twenty HCV patients were treated with P/R for 24-72 weeks, out of them 195 (46.4%) achieved SVR. The IL28 rs12979860 SNP was determined using Custom Taqman SNP Genotyping Assays. The IL10R-1087 (also known as IL10R-1082 (rs1800896) promoter region SNP was determined by RT-PCR and restriction fragment length polymorphism analysis. Results: The IL28B CC genotype occurred with lower frequency in HCV patients than in controls (26.1% vs 51.4%, p

Original languageEnglish
Article number12
JournalBMC Research Notes
Volume7
Issue number1
DOIs
Publication statusPublished - jan. 1 2014

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Polymorphism
Interferons
Single Nucleotide Polymorphism
Ribavirin
Genotype
Nucleotides
Infection
Chronic Hepatitis C
Therapeutics
Blood Donors
Genetic Promoter Regions
Gene Frequency
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Assays
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

IL28B and IL10R-1087 polymorphisms are protective for chronic genotype 1 HCV infection and predictors of response to interferon-based therapy in an East-Central European cohort. / Pár, A.; Pár, G.; Tornai, István; Szalay, F.; Várszegi, Dalma; Fráter, Edit; Papp, M.; Lengyel, G.; Fehér, J.; Varga, Márta; Gervain, Judit; Schuller, János; Nemes, Zsuzsanna; Péterfi, Zoltán; Tusnádi, Anna; Hunyady, B.; Haragh, Attila; Szinku, Zsolt; Vincze, A.; Szereday, L.; Kisfali, P.; Melegh, B.

In: BMC Research Notes, Vol. 7, No. 1, 12, 01.01.2014.

Research output: Article

Pár, A. ; Pár, G. ; Tornai, István ; Szalay, F. ; Várszegi, Dalma ; Fráter, Edit ; Papp, M. ; Lengyel, G. ; Fehér, J. ; Varga, Márta ; Gervain, Judit ; Schuller, János ; Nemes, Zsuzsanna ; Péterfi, Zoltán ; Tusnádi, Anna ; Hunyady, B. ; Haragh, Attila ; Szinku, Zsolt ; Vincze, A. ; Szereday, L. ; Kisfali, P. ; Melegh, B. / IL28B and IL10R-1087 polymorphisms are protective for chronic genotype 1 HCV infection and predictors of response to interferon-based therapy in an East-Central European cohort. In: BMC Research Notes. 2014 ; Vol. 7, No. 1.
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abstract = "Background: Previous studies have shown that single nucleotide polymorphisms (SNP) in IL28B and IL10R are associated with sustained virological response (SVR) in chronic hepatitis C patients treated with pegilated interferon plus ribavirin (P/R). The present study extends our earlier investigations on a large East-Central European cohort. The allele frequencies of IL28B and IL10R in genotype 1 HCV infection were compared with that of healthy controls for the purpose of examining the relationship between the polymorphisms and the SVR to P/R treatment. Methods: A total of 748 chronic HCV1 infected patients (365 male, 383 female; 18-82 years) and 105 voluntary blood donors as controls were enrolled. Four hundred and twenty HCV patients were treated with P/R for 24-72 weeks, out of them 195 (46.4{\%}) achieved SVR. The IL28 rs12979860 SNP was determined using Custom Taqman SNP Genotyping Assays. The IL10R-1087 (also known as IL10R-1082 (rs1800896) promoter region SNP was determined by RT-PCR and restriction fragment length polymorphism analysis. Results: The IL28B CC genotype occurred with lower frequency in HCV patients than in controls (26.1{\%} vs 51.4{\%}, p",
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T1 - IL28B and IL10R-1087 polymorphisms are protective for chronic genotype 1 HCV infection and predictors of response to interferon-based therapy in an East-Central European cohort

AU - Pár, A.

AU - Pár, G.

AU - Tornai, István

AU - Szalay, F.

AU - Várszegi, Dalma

AU - Fráter, Edit

AU - Papp, M.

AU - Lengyel, G.

AU - Fehér, J.

AU - Varga, Márta

AU - Gervain, Judit

AU - Schuller, János

AU - Nemes, Zsuzsanna

AU - Péterfi, Zoltán

AU - Tusnádi, Anna

AU - Hunyady, B.

AU - Haragh, Attila

AU - Szinku, Zsolt

AU - Vincze, A.

AU - Szereday, L.

AU - Kisfali, P.

AU - Melegh, B.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Previous studies have shown that single nucleotide polymorphisms (SNP) in IL28B and IL10R are associated with sustained virological response (SVR) in chronic hepatitis C patients treated with pegilated interferon plus ribavirin (P/R). The present study extends our earlier investigations on a large East-Central European cohort. The allele frequencies of IL28B and IL10R in genotype 1 HCV infection were compared with that of healthy controls for the purpose of examining the relationship between the polymorphisms and the SVR to P/R treatment. Methods: A total of 748 chronic HCV1 infected patients (365 male, 383 female; 18-82 years) and 105 voluntary blood donors as controls were enrolled. Four hundred and twenty HCV patients were treated with P/R for 24-72 weeks, out of them 195 (46.4%) achieved SVR. The IL28 rs12979860 SNP was determined using Custom Taqman SNP Genotyping Assays. The IL10R-1087 (also known as IL10R-1082 (rs1800896) promoter region SNP was determined by RT-PCR and restriction fragment length polymorphism analysis. Results: The IL28B CC genotype occurred with lower frequency in HCV patients than in controls (26.1% vs 51.4%, p

AB - Background: Previous studies have shown that single nucleotide polymorphisms (SNP) in IL28B and IL10R are associated with sustained virological response (SVR) in chronic hepatitis C patients treated with pegilated interferon plus ribavirin (P/R). The present study extends our earlier investigations on a large East-Central European cohort. The allele frequencies of IL28B and IL10R in genotype 1 HCV infection were compared with that of healthy controls for the purpose of examining the relationship between the polymorphisms and the SVR to P/R treatment. Methods: A total of 748 chronic HCV1 infected patients (365 male, 383 female; 18-82 years) and 105 voluntary blood donors as controls were enrolled. Four hundred and twenty HCV patients were treated with P/R for 24-72 weeks, out of them 195 (46.4%) achieved SVR. The IL28 rs12979860 SNP was determined using Custom Taqman SNP Genotyping Assays. The IL10R-1087 (also known as IL10R-1082 (rs1800896) promoter region SNP was determined by RT-PCR and restriction fragment length polymorphism analysis. Results: The IL28B CC genotype occurred with lower frequency in HCV patients than in controls (26.1% vs 51.4%, p

KW - Genetic polymorphism

KW - Hepatitis C virus

KW - Interferon

KW - Interleukin-10

KW - Interleukin-28B

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DO - 10.1186/1756-0500-7-12

M3 - Article

C2 - 24398031

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VL - 7

JO - BMC Research Notes

JF - BMC Research Notes

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