Identification of silymarin constituents: An improved HPLC-MS method

Ákos Kuki, Lajos Nagy, G. Deák, Miklós Nagy, M. Zsuga, S. Kéki

Research output: Article

27 Citations (Scopus)

Abstract

A high-performance liquid chromatographic/tandem mass spectrometric method was developed for the determination of the major bioactive flavonolignans in silymarin, a herbal remedy extracted from the milk thistle Silybum marianum. In this study, eight active components of silymarin with the same elemental composition, including silychristins A and B, silydianin, silybin A and B, isosilybin A and B and an unknown compound were completely separated. Furthermore, three additional components were detected and partly separated; presumably two silybin stereoisomers and one isosilybin stereoisomer. The collision-induced dissociation (CID) MS/MS spectra of these silymarin constituents were studied: the spectral similarity values of the component pairs were determined, and simple criteria were found for distinguishing the components.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalChromatographia
Volume75
Issue number3-4
DOIs
Publication statusPublished - febr. 2012

Fingerprint

Silymarin
Milk Thistle
Stereoisomerism
High Pressure Liquid Chromatography
Flavonolignans
Liquids
Chemical analysis
isosilybin A
silybin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Identification of silymarin constituents : An improved HPLC-MS method. / Kuki, Ákos; Nagy, Lajos; Deák, G.; Nagy, Miklós; Zsuga, M.; Kéki, S.

In: Chromatographia, Vol. 75, No. 3-4, 02.2012, p. 175-180.

Research output: Article

Kuki, Ákos ; Nagy, Lajos ; Deák, G. ; Nagy, Miklós ; Zsuga, M. ; Kéki, S. / Identification of silymarin constituents : An improved HPLC-MS method. In: Chromatographia. 2012 ; Vol. 75, No. 3-4. pp. 175-180.
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