Identification of silymarin constituents: An improved HPLC-MS method

Ákos Kuki, Lajos Nagy, György Deák, Miklós Nagy, Miklós Zsuga, Sándor Kéki

Research output: Article

29 Citations (Scopus)


A high-performance liquid chromatographic/tandem mass spectrometric method was developed for the determination of the major bioactive flavonolignans in silymarin, a herbal remedy extracted from the milk thistle Silybum marianum. In this study, eight active components of silymarin with the same elemental composition, including silychristins A and B, silydianin, silybin A and B, isosilybin A and B and an unknown compound were completely separated. Furthermore, three additional components were detected and partly separated; presumably two silybin stereoisomers and one isosilybin stereoisomer. The collision-induced dissociation (CID) MS/MS spectra of these silymarin constituents were studied: the spectral similarity values of the component pairs were determined, and simple criteria were found for distinguishing the components.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
Issue number3-4
Publication statusPublished - febr. 1 2012


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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