Identification and isolation of new neolignan and sesquineolignan species: Their acid-catalyzed ring closure and specific accumulation in the fruit wall of Cirsium eriophorum (L.) Scop.

Anna Sólyomváry, Gergo Tóth, Balázs Komjáti, Péter Horváth, Márta Kraszni, Béla Noszál, Ibolya Molnár-Perl, Imre Boldizsár

Research output: Article

7 Citations (Scopus)

Abstract

In Cirsium eriophorum fruit, the main neolignan and sesquineolignan compounds, denominated prebalanophonin (preBA) and prepicrasmalignan (prePI), were determined for the first time in the plant kingdom using a combination of optimized acid treatments and complementary spectroscopic (HPLC-MS, GC-MS, CD and NMR) methods. Analysis of fruit parts separated via germination, demonstrated the specific accumulation of these compounds, since preBA and prePI were exclusively found in the fruit wall. Based on quantitative approaches obtained by HPLC-UV measurements, the fruit wall was found to contain extraordinarily high amounts of preBA (4.57%) and prePI (2.88%) allowing their high-yield isolation by preparative HPLC. Optimized acidic treatment (2 N trifluoroacetic acid, 50°C, 15 min) of the wall extract resulted in the quantitative transformation of preBA and prePI into balanophonin (BA) and picrasmalignan (PI), as a result of acid-catalyzed cyclization by the SN2 reaction. Consequently, acid-treated wall extract was found to be the richest raw material containing BA and PI (5.3% and 3.10%), reported to date.

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalProcess Biochemistry
Volume50
Issue number5
DOIs
Publication statusPublished - máj. 1 2015

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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