HPLC determination of carotenoids in human serum by gradient elution technique

R. Ohmacht, G. Tóth, G. Voigt

Research output: Article

6 Citations (Scopus)

Abstract

Polarities of the carotenoids in human serum are very different; many nonpolar carotenoid hydrocarbons (e.g. β-carotene, lycopene) and highly polar hydroxycarotenoids (e. g. β-cryptoxanthin, zeaxanthin, lutein) can be found among them. Gradient elution chromatography was used for the separation of β-carotene, lycopene, β-cryptoxanthin, lutein and zeaxanthin in serum samples applying amino and cyano packings (Chromsil-NH2, Chromsil-CN). The effects of different stationary phases on the selectivity were compared. The method is particularly suitable for the direct determination of serum levels of carotenoid in serum extracts.

Original languageEnglish
Pages (from-to)189-190
Number of pages2
JournalChromatographia
Volume22
Issue number1-6
DOIs
Publication statusPublished - jún. 1986

Fingerprint

Carotenoids
High Pressure Liquid Chromatography
Lutein
Serum
Hydrocarbons
Chromatography
lycopene
Cryptoxanthins
Zeaxanthins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

HPLC determination of carotenoids in human serum by gradient elution technique. / Ohmacht, R.; Tóth, G.; Voigt, G.

In: Chromatographia, Vol. 22, No. 1-6, 06.1986, p. 189-190.

Research output: Article

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