HPLC analysis of blood-brain barrier penetration of 4-fluorodeprenyl

Zita Pöstényi, Kornélia Tekes, Edit Tóth-Molnár, Huba Kalász

Research output: Article

4 Citations (Scopus)

Abstract

Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30. mg/kg of (-)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60. min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24. μg/g, showing maximum concentration in the brain and the liver after 5. min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15. min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume102
DOIs
Publication statusPublished - jan. 5 2015

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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