How do methanol and higher alcohols found in alcoholic beverages affect membrane fluidity and migration of granulocytes?

László Pál, Orsolya Bujdosó, Sándor Szűcs, Gergő Baranyi, Veronika Sebestyén, G. Vámosi, Gábor Rácz, R. Ádány, Martin McKee, Ervin M. Árnyas

Research output: Article

Abstract

Ethanol-induced changes in membrane fluidity and migration of granulocytes has been suggested to contribute to development of alcoholic hepatitis. Besides ethanol other aliphatic alcohols (OAAs) have been detected in alcoholic beverages. We examined the effects of OAAs alone and in combination with ethanol on these processes. Granulocytes isolated from human blood were treated separately with methanol, ethanol, and a mixture of OAAs including methanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, isobutanol, and isoamyl alcohol at biologically relevant concentrations. The membrane fluidity and migration activity of granulocytes was examined by fluorescence anisotropy method and migration chambers, respectively. Our findings showed that OAAs increased membrane fluidity and migration activity of granulocytes in a concentration-dependent manner and when combined with ethanol they caused a further increase in the migration activity of granulocytes. It is possible that OAAs consumed with alcoholic beverages may further enhance migration of granulocytes in heavy drinkers and contribute to ethanol-induced liver damage. Practical applications. The consumption of OAAs in alcoholic beverages has been hypothesized as an additional risk factor for the development of alcoholic liver diseases. To confirm this suggestion, the potentially adverse effects of exposure to OAAs should be further examined. Our investigation provides new toxicological data on the effects of OAAs found in alcoholic beverages. The results of this study can be used for more precise toxicological evaluations to clarify whether OAAs may pose an additional risk factor for the development of alcoholic liver diseases. Furthermore, information on the harmful effects of OAAs could enable health policy makers to make evidence-based decisions when implementing new regulations on alcoholic beverages.

Original languageEnglish
JournalJournal of Food Biochemistry
DOIs
Publication statusAccepted/In press - jan. 1 2018

Fingerprint

Alcoholic Beverages
membrane fluidity
Membrane Fluidity
alcoholic beverages
granulocytes
Granulocytes
Methanol
methanol
alcohols
Alcohols
Ethanol
ethanol
Alcoholic Liver Diseases
liver diseases
alcohol abuse
butanol
Toxicology
alcoholic hepatitis
risk factors
isoamyl alcohol

ASJC Scopus subject areas

  • Biophysics
  • Food Science
  • Pharmacology
  • Cell Biology

Cite this

How do methanol and higher alcohols found in alcoholic beverages affect membrane fluidity and migration of granulocytes? / Pál, László; Bujdosó, Orsolya; Szűcs, Sándor; Baranyi, Gergő; Sebestyén, Veronika; Vámosi, G.; Rácz, Gábor; Ádány, R.; McKee, Martin; Árnyas, Ervin M.

In: Journal of Food Biochemistry, 01.01.2018.

Research output: Article

Pál, László ; Bujdosó, Orsolya ; Szűcs, Sándor ; Baranyi, Gergő ; Sebestyén, Veronika ; Vámosi, G. ; Rácz, Gábor ; Ádány, R. ; McKee, Martin ; Árnyas, Ervin M. / How do methanol and higher alcohols found in alcoholic beverages affect membrane fluidity and migration of granulocytes?. In: Journal of Food Biochemistry. 2018.
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abstract = "Ethanol-induced changes in membrane fluidity and migration of granulocytes has been suggested to contribute to development of alcoholic hepatitis. Besides ethanol other aliphatic alcohols (OAAs) have been detected in alcoholic beverages. We examined the effects of OAAs alone and in combination with ethanol on these processes. Granulocytes isolated from human blood were treated separately with methanol, ethanol, and a mixture of OAAs including methanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, isobutanol, and isoamyl alcohol at biologically relevant concentrations. The membrane fluidity and migration activity of granulocytes was examined by fluorescence anisotropy method and migration chambers, respectively. Our findings showed that OAAs increased membrane fluidity and migration activity of granulocytes in a concentration-dependent manner and when combined with ethanol they caused a further increase in the migration activity of granulocytes. It is possible that OAAs consumed with alcoholic beverages may further enhance migration of granulocytes in heavy drinkers and contribute to ethanol-induced liver damage. Practical applications. The consumption of OAAs in alcoholic beverages has been hypothesized as an additional risk factor for the development of alcoholic liver diseases. To confirm this suggestion, the potentially adverse effects of exposure to OAAs should be further examined. Our investigation provides new toxicological data on the effects of OAAs found in alcoholic beverages. The results of this study can be used for more precise toxicological evaluations to clarify whether OAAs may pose an additional risk factor for the development of alcoholic liver diseases. Furthermore, information on the harmful effects of OAAs could enable health policy makers to make evidence-based decisions when implementing new regulations on alcoholic beverages.",
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AU - Pál, László

AU - Bujdosó, Orsolya

AU - Szűcs, Sándor

AU - Baranyi, Gergő

AU - Sebestyén, Veronika

AU - Vámosi, G.

AU - Rácz, Gábor

AU - Ádány, R.

AU - McKee, Martin

AU - Árnyas, Ervin M.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Ethanol-induced changes in membrane fluidity and migration of granulocytes has been suggested to contribute to development of alcoholic hepatitis. Besides ethanol other aliphatic alcohols (OAAs) have been detected in alcoholic beverages. We examined the effects of OAAs alone and in combination with ethanol on these processes. Granulocytes isolated from human blood were treated separately with methanol, ethanol, and a mixture of OAAs including methanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, isobutanol, and isoamyl alcohol at biologically relevant concentrations. The membrane fluidity and migration activity of granulocytes was examined by fluorescence anisotropy method and migration chambers, respectively. Our findings showed that OAAs increased membrane fluidity and migration activity of granulocytes in a concentration-dependent manner and when combined with ethanol they caused a further increase in the migration activity of granulocytes. It is possible that OAAs consumed with alcoholic beverages may further enhance migration of granulocytes in heavy drinkers and contribute to ethanol-induced liver damage. Practical applications. The consumption of OAAs in alcoholic beverages has been hypothesized as an additional risk factor for the development of alcoholic liver diseases. To confirm this suggestion, the potentially adverse effects of exposure to OAAs should be further examined. Our investigation provides new toxicological data on the effects of OAAs found in alcoholic beverages. The results of this study can be used for more precise toxicological evaluations to clarify whether OAAs may pose an additional risk factor for the development of alcoholic liver diseases. Furthermore, information on the harmful effects of OAAs could enable health policy makers to make evidence-based decisions when implementing new regulations on alcoholic beverages.

AB - Ethanol-induced changes in membrane fluidity and migration of granulocytes has been suggested to contribute to development of alcoholic hepatitis. Besides ethanol other aliphatic alcohols (OAAs) have been detected in alcoholic beverages. We examined the effects of OAAs alone and in combination with ethanol on these processes. Granulocytes isolated from human blood were treated separately with methanol, ethanol, and a mixture of OAAs including methanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, isobutanol, and isoamyl alcohol at biologically relevant concentrations. The membrane fluidity and migration activity of granulocytes was examined by fluorescence anisotropy method and migration chambers, respectively. Our findings showed that OAAs increased membrane fluidity and migration activity of granulocytes in a concentration-dependent manner and when combined with ethanol they caused a further increase in the migration activity of granulocytes. It is possible that OAAs consumed with alcoholic beverages may further enhance migration of granulocytes in heavy drinkers and contribute to ethanol-induced liver damage. Practical applications. The consumption of OAAs in alcoholic beverages has been hypothesized as an additional risk factor for the development of alcoholic liver diseases. To confirm this suggestion, the potentially adverse effects of exposure to OAAs should be further examined. Our investigation provides new toxicological data on the effects of OAAs found in alcoholic beverages. The results of this study can be used for more precise toxicological evaluations to clarify whether OAAs may pose an additional risk factor for the development of alcoholic liver diseases. Furthermore, information on the harmful effects of OAAs could enable health policy makers to make evidence-based decisions when implementing new regulations on alcoholic beverages.

KW - alcohol consumption

KW - alcoholic liver damage

KW - aliphatic alcohols

KW - granulocytes

KW - membrane fluidity

KW - membrane fluidity

KW - migration

KW - migration

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