Hodgkin-lymphoma-derived cells show high sensitivity to dactinomycin and paclitaxel

Laszlo Markasz, Lorand Levente Kis, Gyorgy Stuber, Emilie Flaberg, Rita Otvos, Staffan Eksborg, Henriette Skribek, Eva Olah, Laszlo Szekely

Research output: Article

12 Citations (Scopus)

Abstract

Depending on stage and risk factors, up to 30% of patients with advanced Hodgkin lymphoma (HL) progress or relapse. Patients with pleural effusions have a particularly poor prognosis and this stage of HL is regularly resistant to chemotherapy. All currently available HL cell lines are derived from late stage HL patients. In the present study we measured the sensitivity of these HL lines against the 26 most frequently used cytostatic drugs. We used a novel fluorescent short-term survival assay where the cell was incubated with the drugs for 4 days. The precise number of differentially stained live and dead cells was determined using a custom-built automated laser confocal fluorescent microscope. We found that HL cells, independently of their origin, showed very similar sensitivity patterns for several of the drugs. All HL cell lines were highly sensitive to dactinomycin, paclitaxel and etoposide. Our data suggest that the inclusion of dactinomycin and paclitaxel into chemotherapy protocols against late stage Hodgkin lymphoma with pleural effusion may be justified.

Original languageEnglish
Pages (from-to)1835-1845
Number of pages11
JournalLeukemia and Lymphoma
Volume48
Issue number9
DOIs
Publication statusPublished - szept. 1 2007

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Markasz, L., Kis, L. L., Stuber, G., Flaberg, E., Otvos, R., Eksborg, S., Skribek, H., Olah, E., & Szekely, L. (2007). Hodgkin-lymphoma-derived cells show high sensitivity to dactinomycin and paclitaxel. Leukemia and Lymphoma, 48(9), 1835-1845. https://doi.org/10.1080/10428190701559132