Hippocampal pyramidal cells excite inhibitory neurons through a single release site

A. I. Gulyás, R. Miles, A. Sík, K. Tóth, N. Tamamaki, T. Freund

Research output: Article

262 Citations (Scopus)

Abstract

MORPHOLOGICALLY a synapse consists of a presynaptic release site containing vesicles, a postsynaptic element with membrane specialization, and a synaptic cleft between them1. The number of release sites shapes the properties of synaptic transmission between neurons2-4. Although excitatory interactions between cortical neurons have been examined5-9, the number of release sites remains unknown. We have now recorded excitatory postsynaptic potentials evoked by single pyramidal cells in hippocampal interneurons and visualized both cells using biocytin injections. Light and electron microscopy showed that excitatory postsynaptic potentials were mediated by a single synapse. We also reconstructed the entire axon arborization of single pyramidal cells, filled in vivo, in sections counterstained for parvalbumin, which selectively marks basket and axo-axonic cells10,11. Single synaptic contacts between pyramidal cells and parvalbumin-containing neurons were dominant (>80%), providing evidence for high convergence and divergence in hippocampal networks12.

Original languageEnglish
Pages (from-to)683-687
Number of pages5
JournalNature
Volume366
Issue number6456
Publication statusPublished - dec. 16 1993

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Pyramidal Cells
Parvalbumins
Excitatory Postsynaptic Potentials
Neurons
Synapses
Interneurons
Synaptic Transmission
Axons
Electron Microscopy
Light
Injections
Membranes

ASJC Scopus subject areas

  • General

Cite this

Gulyás, A. I., Miles, R., Sík, A., Tóth, K., Tamamaki, N., & Freund, T. (1993). Hippocampal pyramidal cells excite inhibitory neurons through a single release site. Nature, 366(6456), 683-687.

Hippocampal pyramidal cells excite inhibitory neurons through a single release site. / Gulyás, A. I.; Miles, R.; Sík, A.; Tóth, K.; Tamamaki, N.; Freund, T.

In: Nature, Vol. 366, No. 6456, 16.12.1993, p. 683-687.

Research output: Article

Gulyás, AI, Miles, R, Sík, A, Tóth, K, Tamamaki, N & Freund, T 1993, 'Hippocampal pyramidal cells excite inhibitory neurons through a single release site', Nature, vol. 366, no. 6456, pp. 683-687.
Gulyás AI, Miles R, Sík A, Tóth K, Tamamaki N, Freund T. Hippocampal pyramidal cells excite inhibitory neurons through a single release site. Nature. 1993 dec. 16;366(6456):683-687.
Gulyás, A. I. ; Miles, R. ; Sík, A. ; Tóth, K. ; Tamamaki, N. ; Freund, T. / Hippocampal pyramidal cells excite inhibitory neurons through a single release site. In: Nature. 1993 ; Vol. 366, No. 6456. pp. 683-687.
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