High rate of neoplastic cells with genetic abnormalities in proliferation centers of chronic lymphocytic leukemia

Zsófia Balogh, Lilla Reiniger, Hajnalka Rajnai, Judit Csomor, Ágota Szepesi, Anikó Balogh, Linda Deák, Éva Gagyi, Csaba Bödör, András Matolcsy

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13 Citations (Scopus)

Abstract

In lymph nodes, chronic lymphocytic leukemia (CLL) cells (prolymphocytes and paraimmunoblasts) form proliferation centers (PCs), which are also known as pseudofollicles. To reveal whether PCs play a role in the accumulation of genetic alterations in CLL, we compared deletion at 11q22.3, 13q14.3, and 17p13.1 loci and trisomy 12 by the fluorescence in situ hybridization (FISH) technique in PCs versus surrounding small lymphocytes (SLs) in 12 formalin-fixed paraffin-embedded (FFPE) lymph nodes. The FFPE sections were stained with methylene blue and PCs were marked by laser beam. Subsequent FISH analysis was performed, relocalizing the previously defined regions. Loss of 11q was detected in five cases, loss of 13q in two cases, loss of 17p in two cases, and trisomy 12 in one case. In seven cases PCs contained a significantly higher ratio of cells with genetic alterations compared with the surrounding SLs. Our results show that CLL cells with genetic alterations tend to accumulate in PCs. The clonal expansion of the cell population carrying genetic alterations within PCs may contribute to CLL progression.

Original languageEnglish
Pages (from-to)1080-1084
Number of pages5
JournalLeukemia and Lymphoma
Volume52
Issue number6
DOIs
Publication statusPublished - jún. 1 2011

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ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Balogh, Z., Reiniger, L., Rajnai, H., Csomor, J., Szepesi, Á., Balogh, A., Deák, L., Gagyi, É., Bödör, C., & Matolcsy, A. (2011). High rate of neoplastic cells with genetic abnormalities in proliferation centers of chronic lymphocytic leukemia. Leukemia and Lymphoma, 52(6), 1080-1084. https://doi.org/10.3109/10428194.2011.555889