High-performance liquid chromatographic detection of side reactions in peptide synthesis

Gyula Szókán, Angela Török, Botond Penke

Research output: Article

9 Citations (Scopus)


High-performance liquid chromatographic systems with chemically bonded packing materials were elaborated for monitoring synthetic stages during peptide coupling, purity control of peptides produced by solid-phase methods and detection of impurities formed as a consequence of side reactions such as oxidation, desulphation, racemization, transpeptidation, alkylation and decomposition. The technique was applied to the analysis of peptide hormones, neuropeptides, cyclopeptides, isopeptides and branched polypeptides based on poly-lysine. The mobile phases were methanol-acetonitrile-water mixtures containing phosphate, acetate, carbonate buffers or trifluoroacetic acid (pH 2-9). After optimization, baseline separations could be achieved for some critical peptide separations.

Original languageEnglish
Pages (from-to)267-280
Number of pages14
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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