High-level cellular and humoral immune responses in guinea pigs immunized intradermally with a heat-inactivated varicella-zoster virus vaccine

Julia Sarkadi, Mate Jankovics, Kinga Fodor, Zoltan Kis, M. Takács, Ildiko Visontai, Istvan Jankovics, E. Gönczöl

Research output: Article

5 Citations (Scopus)

Abstract

The threat of varicella and herpes zoster in immunocompromised individuals necessitates the development of a safe and effective varicella-zoster virus (VZV) vaccine. The immune responses of guinea pigs to the intradermal (i.d.) or subcutaneous (s.c.) administration of a heat-inactivated or live VZV vaccine were investigated. Relative to nonimmunized animals, a single 399-PFU dose of vaccine induced nonsignificant increases in gamma interferon (IFN-γ), granzyme B, and perforin mRNA expression in the splenocytes of all groups, while two i.d. administrations of the inactivated vaccine increased IFN-γ mRNA expression significantly (P <0.005). A single 1,995-PFU dose significantly increased the expression of IFN-γ mRNA in the groups receiving the vaccine either i.d. (P <0.005) or s.c. (P <0.05), that of granzyme B mRNA in the groups immunized i.d. with the inactivated (P <0.005) or live (P <0.005) vaccine, and that of perforin mRNA in the animals that received the inactivated vaccine i.d. (P <0.005). Importantly, increases in the expression of IFN-γ (P = 0.025), granzyme B (P = 0.004), and perforin (P > 0.05) mRNAs were observed in the animals immunized i.d. with 1,995 PFU of inactivated vaccine relative to those immunized s.c. with the same dose. The proportion of animals expressing IFN-γ mRNA mirrored the proportion expressing IFN-γ protein (correlation coefficient of 0.88). VZV glycoprotein-specific and virus-neutralizing antibodies were produced with no significant intergroup differences. A booster i.d. administration of the 399-PFU dose of heat-inactivated vaccine enhanced the antibody responses. These results demonstrate that i.d. administration of an inactivated VZV vaccine can be an efficient mode of immunization against VZV.

Original languageEnglish
Pages (from-to)570-577
Number of pages8
JournalClinical and Vaccine Immunology
Volume22
Issue number5
DOIs
Publication statusPublished - máj. 1 2015

Fingerprint

Herpes Zoster Vaccine
Human Herpesvirus 3
Humoral Immunity
Viruses
Cellular Immunity
Guinea Pigs
Vaccines
Hot Temperature
Inactivated Vaccines
Interferons
Messenger RNA
Animals
Chickenpox
Immunization
Herpes Zoster
Neutralizing Antibodies
Interferon-gamma
Antibody Formation
Glycoproteins
Antibodies

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

High-level cellular and humoral immune responses in guinea pigs immunized intradermally with a heat-inactivated varicella-zoster virus vaccine. / Sarkadi, Julia; Jankovics, Mate; Fodor, Kinga; Kis, Zoltan; Takács, M.; Visontai, Ildiko; Jankovics, Istvan; Gönczöl, E.

In: Clinical and Vaccine Immunology, Vol. 22, No. 5, 01.05.2015, p. 570-577.

Research output: Article

Sarkadi, Julia ; Jankovics, Mate ; Fodor, Kinga ; Kis, Zoltan ; Takács, M. ; Visontai, Ildiko ; Jankovics, Istvan ; Gönczöl, E. / High-level cellular and humoral immune responses in guinea pigs immunized intradermally with a heat-inactivated varicella-zoster virus vaccine. In: Clinical and Vaccine Immunology. 2015 ; Vol. 22, No. 5. pp. 570-577.
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AU - Fodor, Kinga

AU - Kis, Zoltan

AU - Takács, M.

AU - Visontai, Ildiko

AU - Jankovics, Istvan

AU - Gönczöl, E.

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