Heat-inducible expression of a reporter gene detected by transient assay in zebrafish

Attila Ádám, Richárd Bártfai, Zsolt Lele, Patrick H. Krone, László Orbán

Research output: Article

38 Citations (Scopus)

Abstract

Heat-inducibility of two reporter constructs expressing lacZ gene under the control of mouse and Xenopus hsp70 promoters was tested in zebrafish (Danio rerio) embryos using a transient expression system. Cells expressing β-galactosidase were stained blue by histochemical staining and their average number per embryo was used as an indicator of the expression level of the reporter gene. Both constructs were heat-inducible in the embryonic tissues and showed similar heat dependence (increasing expression levels from 35-36°C up to 39°C with an apparent decrease at 40°C), resembling that of the zebrafish hsp70 genes. However, their induction kinetics were different, which might be due to differences in their 5' UTRs. Spatial expression patterns of the two hsp/lacZ constructs and an endogenous hsp70 gene were mostly similar on the RNA level. These results indicate that our approach is applicable for in vivo analysis of the heat-shock response and that exogenous heat-shock promoters may be useful for inducible expression of transgenes in fish. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)282-290
Number of pages9
JournalExperimental Cell Research
Volume256
Issue number1
DOIs
Publication statusPublished - ápr. 10 2000

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ASJC Scopus subject areas

  • Cell Biology

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