GRP78 upregulation-induced increase in cisplatin sensitivity of SPCA1 lung cancer cells

Li Chuan Zhang, Jia Rui Wang, Long Zhao, Tao Wang, Jing Wu, Su Fang Fan, Li Xia Chen, Shu Juan Shao, J. Molnár, Qi Wang

Research output: Article

4 Citations (Scopus)

Abstract

Background Glucose regulated protein 78 (GRP78), an endoplasmic reticulum (ER) chaperone, plays a critical role in chemotherapy resistance in a variety of cancers. In this study, we investigated the up-regulation of GRP78 induced by A23187 and its association with the chemotherapeutical sensibility to cisplatin in human lung cancer cell line SPCA1. Methods SPCA1 cells were pretreated with A23187 at different concentrations. The expression of GRP78 at the mRNA level was analyzed by RT-PCR; the expression of GRP78 at the protein level was determined by Western blotting and immunofluorescence assay. Cell survival was determined by MTT assay. Cell apoptosis was analyzed by flow cytometry. Results The expression of GRP78 at both the mRNA and protein levels was obviously induced by A23187 in SPCA1 cells, with an elevation of GRP78 by 2.1-fold at the mRNA level and by 3.8-fold at the protein level compared to the control. There was a dose-dependent response. Survival curve analysis demonstrated that A23187 induction caused a significant reduction of survival for the cells subjected to cisplatin treatment (P

Original languageEnglish
Pages (from-to)3341-3346
Number of pages6
JournalChinese Medical Journal
Volume124
Issue number20
DOIs
Publication statusPublished - nov. 2011

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Cisplatin
Lung Neoplasms
Up-Regulation
Calcimycin
Messenger RNA
Cell Survival
Proteins
Survival Analysis
Endoplasmic Reticulum
Fluorescent Antibody Technique
glucose-regulated proteins
Flow Cytometry
Western Blotting
Apoptosis
Drug Therapy
Cell Line
Polymerase Chain Reaction
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Zhang, L. C., Wang, J. R., Zhao, L., Wang, T., Wu, J., Fan, S. F., ... Wang, Q. (2011). GRP78 upregulation-induced increase in cisplatin sensitivity of SPCA1 lung cancer cells. Chinese Medical Journal, 124(20), 3341-3346. https://doi.org/10.3760/cma.j.issn.0366-6999.2011.20.024

GRP78 upregulation-induced increase in cisplatin sensitivity of SPCA1 lung cancer cells. / Zhang, Li Chuan; Wang, Jia Rui; Zhao, Long; Wang, Tao; Wu, Jing; Fan, Su Fang; Chen, Li Xia; Shao, Shu Juan; Molnár, J.; Wang, Qi.

In: Chinese Medical Journal, Vol. 124, No. 20, 11.2011, p. 3341-3346.

Research output: Article

Zhang, LC, Wang, JR, Zhao, L, Wang, T, Wu, J, Fan, SF, Chen, LX, Shao, SJ, Molnár, J & Wang, Q 2011, 'GRP78 upregulation-induced increase in cisplatin sensitivity of SPCA1 lung cancer cells', Chinese Medical Journal, vol. 124, no. 20, pp. 3341-3346. https://doi.org/10.3760/cma.j.issn.0366-6999.2011.20.024
Zhang, Li Chuan ; Wang, Jia Rui ; Zhao, Long ; Wang, Tao ; Wu, Jing ; Fan, Su Fang ; Chen, Li Xia ; Shao, Shu Juan ; Molnár, J. ; Wang, Qi. / GRP78 upregulation-induced increase in cisplatin sensitivity of SPCA1 lung cancer cells. In: Chinese Medical Journal. 2011 ; Vol. 124, No. 20. pp. 3341-3346.
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T1 - GRP78 upregulation-induced increase in cisplatin sensitivity of SPCA1 lung cancer cells

AU - Zhang, Li Chuan

AU - Wang, Jia Rui

AU - Zhao, Long

AU - Wang, Tao

AU - Wu, Jing

AU - Fan, Su Fang

AU - Chen, Li Xia

AU - Shao, Shu Juan

AU - Molnár, J.

AU - Wang, Qi

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N2 - Background Glucose regulated protein 78 (GRP78), an endoplasmic reticulum (ER) chaperone, plays a critical role in chemotherapy resistance in a variety of cancers. In this study, we investigated the up-regulation of GRP78 induced by A23187 and its association with the chemotherapeutical sensibility to cisplatin in human lung cancer cell line SPCA1. Methods SPCA1 cells were pretreated with A23187 at different concentrations. The expression of GRP78 at the mRNA level was analyzed by RT-PCR; the expression of GRP78 at the protein level was determined by Western blotting and immunofluorescence assay. Cell survival was determined by MTT assay. Cell apoptosis was analyzed by flow cytometry. Results The expression of GRP78 at both the mRNA and protein levels was obviously induced by A23187 in SPCA1 cells, with an elevation of GRP78 by 2.1-fold at the mRNA level and by 3.8-fold at the protein level compared to the control. There was a dose-dependent response. Survival curve analysis demonstrated that A23187 induction caused a significant reduction of survival for the cells subjected to cisplatin treatment (P

AB - Background Glucose regulated protein 78 (GRP78), an endoplasmic reticulum (ER) chaperone, plays a critical role in chemotherapy resistance in a variety of cancers. In this study, we investigated the up-regulation of GRP78 induced by A23187 and its association with the chemotherapeutical sensibility to cisplatin in human lung cancer cell line SPCA1. Methods SPCA1 cells were pretreated with A23187 at different concentrations. The expression of GRP78 at the mRNA level was analyzed by RT-PCR; the expression of GRP78 at the protein level was determined by Western blotting and immunofluorescence assay. Cell survival was determined by MTT assay. Cell apoptosis was analyzed by flow cytometry. Results The expression of GRP78 at both the mRNA and protein levels was obviously induced by A23187 in SPCA1 cells, with an elevation of GRP78 by 2.1-fold at the mRNA level and by 3.8-fold at the protein level compared to the control. There was a dose-dependent response. Survival curve analysis demonstrated that A23187 induction caused a significant reduction of survival for the cells subjected to cisplatin treatment (P

KW - Chemosensitivity

KW - Cisplatin

KW - GRP78

KW - Human lung cancer cell line SPCA1

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