GLP-1 receptor stimulation of the lateral parabrachial nucleus reduces food intake: Neuroanatomical, electrophysiological, and behavioral evidence

Jennifer E. Richard, Imre Farkas, Fredrik Anesten, Rozita H. Anderberg, Suzanne L. Dickson, Fiona M. Gribble, Frank Reimann, John Olov Jansson, Z. Liposits, Karolina P. Skibicka

Research output: Article

43 Citations (Scopus)

Abstract

The parabrachial nucleus (PBN) is a key nucleus for the regulation of feeding behavior. Inhibitory inputs from the hypothalamus to the PBN play a crucial role in the normal maintenance of feeding behavior, because their loss leads to starvation. Viscerosensory stimuli result in neuronal activation of the PBN. However, the origin and neurochemical identity of the excitatory neuronal input to the PBN remain largely unexplored. Here, we hypothesize that hindbrain glucagon-like peptide 1 (GLP-1) neurons provide excitatory inputs to the PBN, activation of which may lead to a reduction in feeding behavior. Our data, obtained from mice expressing the yellow fluorescent protein in GLP-1-producing neurons, revealed that hindbrain GLP-1-producing neurons project to the lateral PBN (lPBN). Stimulation of lPBN GLP-1 receptors (GLP-1Rs) reduced the intake of chow and palatable foodanddecreasedbodyweight in rats. It also activated lPBN neurons, reflected byanincrease in the number of c-Fos-positive cells in this region. Further support for an excitatory role of GLP-1 in the PBN is provided by electrophysiological studies showing a remarkable increase in firing of lPBN neurons after Exendin-4 application. We show that within the PBN, GLP-1R activation increased gene expression of 2 energy balance regulating peptides, calcitonin gene-related peptide (CGRP) and IL-6. Moreover, nearly 70% of the lPBN GLP-1 fibers innervated lPBN CGRP neurons. Direct intra-lPBN CGRP application resulted in anorexia. Collectively, our molecular, anatomical, electrophysiological, pharmacological, and behavioral data provide evidence for a functional role of the GLP-1R for feeding control in the PBN.

Original languageEnglish
Pages (from-to)4356-4367
Number of pages12
JournalEndocrinology
Volume155
Issue number11
DOIs
Publication statusPublished - nov. 1 2014

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Eating
Glucagon-Like Peptide 1
Neurons
Calcitonin Gene-Related Peptide
Feeding Behavior
Rhombencephalon
Glucagon-Like Peptide-1 Receptor
Parabrachial Nucleus
Anorexia
Starvation
Hypothalamus
Interleukin-6
Maintenance
Pharmacology
Gene Expression
Peptides
Proteins

ASJC Scopus subject areas

  • Endocrinology

Cite this

Richard, J. E., Farkas, I., Anesten, F., Anderberg, R. H., Dickson, S. L., Gribble, F. M., ... Skibicka, K. P. (2014). GLP-1 receptor stimulation of the lateral parabrachial nucleus reduces food intake: Neuroanatomical, electrophysiological, and behavioral evidence. Endocrinology, 155(11), 4356-4367. https://doi.org/10.1210/en.2014-1248

GLP-1 receptor stimulation of the lateral parabrachial nucleus reduces food intake : Neuroanatomical, electrophysiological, and behavioral evidence. / Richard, Jennifer E.; Farkas, Imre; Anesten, Fredrik; Anderberg, Rozita H.; Dickson, Suzanne L.; Gribble, Fiona M.; Reimann, Frank; Jansson, John Olov; Liposits, Z.; Skibicka, Karolina P.

In: Endocrinology, Vol. 155, No. 11, 01.11.2014, p. 4356-4367.

Research output: Article

Richard, JE, Farkas, I, Anesten, F, Anderberg, RH, Dickson, SL, Gribble, FM, Reimann, F, Jansson, JO, Liposits, Z & Skibicka, KP 2014, 'GLP-1 receptor stimulation of the lateral parabrachial nucleus reduces food intake: Neuroanatomical, electrophysiological, and behavioral evidence', Endocrinology, vol. 155, no. 11, pp. 4356-4367. https://doi.org/10.1210/en.2014-1248
Richard, Jennifer E. ; Farkas, Imre ; Anesten, Fredrik ; Anderberg, Rozita H. ; Dickson, Suzanne L. ; Gribble, Fiona M. ; Reimann, Frank ; Jansson, John Olov ; Liposits, Z. ; Skibicka, Karolina P. / GLP-1 receptor stimulation of the lateral parabrachial nucleus reduces food intake : Neuroanatomical, electrophysiological, and behavioral evidence. In: Endocrinology. 2014 ; Vol. 155, No. 11. pp. 4356-4367.
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abstract = "The parabrachial nucleus (PBN) is a key nucleus for the regulation of feeding behavior. Inhibitory inputs from the hypothalamus to the PBN play a crucial role in the normal maintenance of feeding behavior, because their loss leads to starvation. Viscerosensory stimuli result in neuronal activation of the PBN. However, the origin and neurochemical identity of the excitatory neuronal input to the PBN remain largely unexplored. Here, we hypothesize that hindbrain glucagon-like peptide 1 (GLP-1) neurons provide excitatory inputs to the PBN, activation of which may lead to a reduction in feeding behavior. Our data, obtained from mice expressing the yellow fluorescent protein in GLP-1-producing neurons, revealed that hindbrain GLP-1-producing neurons project to the lateral PBN (lPBN). Stimulation of lPBN GLP-1 receptors (GLP-1Rs) reduced the intake of chow and palatable foodanddecreasedbodyweight in rats. It also activated lPBN neurons, reflected byanincrease in the number of c-Fos-positive cells in this region. Further support for an excitatory role of GLP-1 in the PBN is provided by electrophysiological studies showing a remarkable increase in firing of lPBN neurons after Exendin-4 application. We show that within the PBN, GLP-1R activation increased gene expression of 2 energy balance regulating peptides, calcitonin gene-related peptide (CGRP) and IL-6. Moreover, nearly 70{\%} of the lPBN GLP-1 fibers innervated lPBN CGRP neurons. Direct intra-lPBN CGRP application resulted in anorexia. Collectively, our molecular, anatomical, electrophysiological, pharmacological, and behavioral data provide evidence for a functional role of the GLP-1R for feeding control in the PBN.",
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