Glomerulosclerosis in transgenic rabbits with ubiquitous venus protein expression

Nándor Lipták, Orsolya Ivett Hoffmann, Gabriella Skoda, Elen Gócza, Andrea Kerekes, Z. Bösze, László Hiripi

Research output: Article

1 Citation (Scopus)

Abstract

Focal segmental glomerulosclerosis (FSGS) is a potential cause of nephrotic syndrome both in humans and pet mammals. Glomerulopathy was reported earlier in green fluorescent protein (GFP) transgenic (TG) mice, but glomerulosclerosis has not been examined in GFP TG rabbits so far. In the present study, the potential manifestation of FSGS was investigated in both Venus TG rabbits generated by Sleeping Beauty (SB) transposition and age-matched control New Zealand White (NZW) rabbits. Venus protein fluorescence was detected by confocal microscopy and quantified by microplate reader. Urinalysis, haematology, serum biochemistry and renal histology were performed to assess the signs of FSGS. Higher levels of Venus fluorescence were determined in renal cortex samples than in the myocardium by both methods. Urinalysis revealed proteinuria in Venus heterozygote TG bucks, while Venus homozygote TG bucks developed microscopic haematuria. Supporting the urinalysis data, the histological findings of FSGS (glomerulomegaly and sclerotic glomeruli) were observed in renal cortex sections of Venus TG rabbits. Taken together, Venus TG bucks were diagnosed with FSGS; thus, this type of glomerulopathy could be a common disease in TG animals overexpressing GFP.

Original languageEnglish
Pages (from-to)281-293
Number of pages13
JournalActa Veterinaria Hungarica
Volume66
Issue number2
DOIs
Publication statusPublished - jún. 1 2018

Fingerprint

Venus
Focal Segmental Glomerulosclerosis
protein synthesis
rabbits
genetically modified organisms
Rabbits
Urinalysis
urinalysis
bucks
Green Fluorescent Proteins
glomerulopathy
green fluorescent protein
Proteins
kidneys
Kidney
cortex
Fluorescence
nephrotic syndrome
fluorescence
Beauty

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Lipták, N., Hoffmann, O. I., Skoda, G., Gócza, E., Kerekes, A., Bösze, Z., & Hiripi, L. (2018). Glomerulosclerosis in transgenic rabbits with ubiquitous venus protein expression. Acta Veterinaria Hungarica, 66(2), 281-293. https://doi.org/10.1556/004.2018.026

Glomerulosclerosis in transgenic rabbits with ubiquitous venus protein expression. / Lipták, Nándor; Hoffmann, Orsolya Ivett; Skoda, Gabriella; Gócza, Elen; Kerekes, Andrea; Bösze, Z.; Hiripi, László.

In: Acta Veterinaria Hungarica, Vol. 66, No. 2, 01.06.2018, p. 281-293.

Research output: Article

Lipták, N, Hoffmann, OI, Skoda, G, Gócza, E, Kerekes, A, Bösze, Z & Hiripi, L 2018, 'Glomerulosclerosis in transgenic rabbits with ubiquitous venus protein expression', Acta Veterinaria Hungarica, vol. 66, no. 2, pp. 281-293. https://doi.org/10.1556/004.2018.026
Lipták, Nándor ; Hoffmann, Orsolya Ivett ; Skoda, Gabriella ; Gócza, Elen ; Kerekes, Andrea ; Bösze, Z. ; Hiripi, László. / Glomerulosclerosis in transgenic rabbits with ubiquitous venus protein expression. In: Acta Veterinaria Hungarica. 2018 ; Vol. 66, No. 2. pp. 281-293.
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