A CRISPR-Cas9 rendszerrel végzett genomszerkesztés módszere és onkológiai alkalmazásai

Viktória Hudacsek, Balázs Gyõrffy

Research output: Review article

Abstract

Today, we have to investigate the effects of selected mutations and molecular alterations - this is made possible by the new genome editing technologies. In these, either a section of the DNA is deleted or moved, or a targeted mutation is introduced into the cell. Here, we describe in detail the CRISPR-Cas9 genome editing method. The technology is based on a feature used by bacteria to combat recurrent viral infections. At present, commencing a set of modifications, the method is functional and can be applied in eukaryotic cells as well. The method has three major steps: first, the system has to be carefully designed and constructed, then the construct has to be introduced into the target cells by transfection, and finally, the achieved effect has to be functionally validated. The reliability of the method enables multiple applications in oncology, including the detailed and efficient investigation of oncogenes, tumor suppressor genes, chromosomal translocations and other molecular changes. At the moment, available CRISPR-Cas9 protocols enable both in vitro and in vivo application. All these already made CRISPR-Cas9 one of the basic methods required for future-proof oncology research.

Translated title of the contributionGenome engineering using the CRISPR-Cas9 system and applications in cancer research
Original languageHungarian
Pages (from-to)119-127
Number of pages9
JournalMagyar onkologia
Volume62
Issue number2
Publication statusPublished - júl. 20 2018

ASJC Scopus subject areas

  • Medicine(all)

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