Genetic variability of central European isolates of the Fusarium graminearum species complex

Beáta Tóth, Ákos Mesterházy, Zoltán Horváth, T. Bartók, Mónika Varga, J. Varga

Research output: Article

65 Citations (Scopus)

Abstract

The main causative agents of Fusarium head blight in central Europe are Fusarium graminearum and F. culmorum. We examined the mycotoxin producing ability, aggressiveness and molecular variability of F. graminearum isolates. Altogether twenty-six Hungarian, three Austrian isolates and representatives of eight species identified in the F. graminearum species complex were involved in this study. Mycotoxin producing abilities of the isolates were tested by GC-MS and HPLC. The central European isolates were found to belong to chemotype I (producing deoxynivalenol). Most isolates produced more 15-acetyl-deoxynivalenol than 3-acetyl-deoxynivalenol suggesting that they belong to chemotype Ib. All F. graminearum isolates were found to be highly pathogenic in in vitro aggressiveness tests. Phylogenetic analysis of random amplified polymorphic DNA profiles, and restriction profiles of the intergenic spacer region of the ribosomal RNA gene cluster of the isolates allowed clustering of the central European isolates into 17 and 16 haplotypes, respectively. When RAPD and IGS-RFLP data were combined, almost every single central European F. graminearum isolate could be differentiated (27/29 haplotypes). Sequence analysis of a putative reductase gene of some isolates was also performed. Based on molecular data, the majority of the central European isolates belonged to F. graminearum sensu stricto characteristic to the northern hemisphere, with the exception of one Hungarian isolate, which was not related to any known species of the F. graminearum species complex based on sequence data. The taxonomic assignment of two other Hungarian isolates, previously suggested as belonging to F. boothii based on mitochondrial DNA restriction profiles, was supported by sequence analysis.

Original languageEnglish
Pages (from-to)35-45
Number of pages11
JournalEuropean Journal of Plant Pathology
Volume113
Issue number1
DOIs
Publication statusPublished - szept. 2005

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Fusarium graminearum
genetic variation
chemotypes
mycotoxins
aggression
haplotypes
sequence analysis
15-acetyldeoxynivalenol
3-acetyldeoxynivalenol
random amplified polymorphic DNA technique
Fusarium culmorum
Fusarium head blight
deoxynivalenol
intergenic DNA
multigene family
Central European region
restriction fragment length polymorphism
mitochondrial DNA
ribosomal RNA
phylogeny

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cite this

Genetic variability of central European isolates of the Fusarium graminearum species complex. / Tóth, Beáta; Mesterházy, Ákos; Horváth, Zoltán; Bartók, T.; Varga, Mónika; Varga, J.

In: European Journal of Plant Pathology, Vol. 113, No. 1, 09.2005, p. 35-45.

Research output: Article

Tóth, Beáta ; Mesterházy, Ákos ; Horváth, Zoltán ; Bartók, T. ; Varga, Mónika ; Varga, J. / Genetic variability of central European isolates of the Fusarium graminearum species complex. In: European Journal of Plant Pathology. 2005 ; Vol. 113, No. 1. pp. 35-45.
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