Gene expression patterns of the Bcl-2 and Bax genes in preterm birth

Csaba Demendi, Balázs Börzsönyi, Veronika Végh, Zsolt B. Nagy, J. Rigó, Attila Pajor, József Gábor Joó

Research output: Article

5 Citations (Scopus)

Abstract

Objective. The apoptotic genes Bax and Bcl-2 are both involved in the pathogenesis of preterm delivery in conjunction with additional factors. We characterized gene expression patterns of these apoptotic regulatory genes as well as relevant environmental factors. Design. A gene expression study with evaluation of clinical data. Setting. Semmelweis University, Budapest, Hungary. Sample. Human placental samples from 104 preterm and 140 full-term pregnancies. Methods. Gene tests were performed using real-time PCR to assess gene expression patterns of Bax and Bcl-2 in human placental samples. Clinical data were collected from our computerized database. Main outcome measures. Apoptotic gene expression pattern and clinical information against the background of preterm delivery. Results. In placental samples from preterm delivery pregnancies, expression of the Bcl-2 gene was unchanged, whereas the Bax gene was overexpressed. Placental gene expression of Bax in preterm delivery was dependent on gestational age with gestational weeks 28-32 and 32-36 associated with overexpression, and no overexpression in gestational weeks 24-28. Preterm delivery began with premature rupture of membranes in 70.2% and spontaneous uterine activity in 29.8%. Conclusions. The Bax gene was overexpressed in preterm delivery, whereas expression of the Bcl-2 gene remained unchanged. After the 28th gestational week, apoptosis appears to be a key factor in the pathogenesis of preterm delivery.

Original languageEnglish
Pages (from-to)1212-1217
Number of pages6
JournalActa Obstetricia et Gynecologica Scandinavica
Volume91
Issue number10
DOIs
Publication statusPublished - okt. 2012

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bcl-2 Genes
Premature Birth
Gene Expression
Genes
Pregnancy
Hungary
Regulator Genes
Gestational Age
Real-Time Polymerase Chain Reaction
Rupture
Outcome Assessment (Health Care)
Databases
Apoptosis
Membranes

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Gene expression patterns of the Bcl-2 and Bax genes in preterm birth. / Demendi, Csaba; Börzsönyi, Balázs; Végh, Veronika; Nagy, Zsolt B.; Rigó, J.; Pajor, Attila; Joó, József Gábor.

In: Acta Obstetricia et Gynecologica Scandinavica, Vol. 91, No. 10, 10.2012, p. 1212-1217.

Research output: Article

Demendi, Csaba ; Börzsönyi, Balázs ; Végh, Veronika ; Nagy, Zsolt B. ; Rigó, J. ; Pajor, Attila ; Joó, József Gábor. / Gene expression patterns of the Bcl-2 and Bax genes in preterm birth. In: Acta Obstetricia et Gynecologica Scandinavica. 2012 ; Vol. 91, No. 10. pp. 1212-1217.
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AU - Végh, Veronika

AU - Nagy, Zsolt B.

AU - Rigó, J.

AU - Pajor, Attila

AU - Joó, József Gábor

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N2 - Objective. The apoptotic genes Bax and Bcl-2 are both involved in the pathogenesis of preterm delivery in conjunction with additional factors. We characterized gene expression patterns of these apoptotic regulatory genes as well as relevant environmental factors. Design. A gene expression study with evaluation of clinical data. Setting. Semmelweis University, Budapest, Hungary. Sample. Human placental samples from 104 preterm and 140 full-term pregnancies. Methods. Gene tests were performed using real-time PCR to assess gene expression patterns of Bax and Bcl-2 in human placental samples. Clinical data were collected from our computerized database. Main outcome measures. Apoptotic gene expression pattern and clinical information against the background of preterm delivery. Results. In placental samples from preterm delivery pregnancies, expression of the Bcl-2 gene was unchanged, whereas the Bax gene was overexpressed. Placental gene expression of Bax in preterm delivery was dependent on gestational age with gestational weeks 28-32 and 32-36 associated with overexpression, and no overexpression in gestational weeks 24-28. Preterm delivery began with premature rupture of membranes in 70.2% and spontaneous uterine activity in 29.8%. Conclusions. The Bax gene was overexpressed in preterm delivery, whereas expression of the Bcl-2 gene remained unchanged. After the 28th gestational week, apoptosis appears to be a key factor in the pathogenesis of preterm delivery.

AB - Objective. The apoptotic genes Bax and Bcl-2 are both involved in the pathogenesis of preterm delivery in conjunction with additional factors. We characterized gene expression patterns of these apoptotic regulatory genes as well as relevant environmental factors. Design. A gene expression study with evaluation of clinical data. Setting. Semmelweis University, Budapest, Hungary. Sample. Human placental samples from 104 preterm and 140 full-term pregnancies. Methods. Gene tests were performed using real-time PCR to assess gene expression patterns of Bax and Bcl-2 in human placental samples. Clinical data were collected from our computerized database. Main outcome measures. Apoptotic gene expression pattern and clinical information against the background of preterm delivery. Results. In placental samples from preterm delivery pregnancies, expression of the Bcl-2 gene was unchanged, whereas the Bax gene was overexpressed. Placental gene expression of Bax in preterm delivery was dependent on gestational age with gestational weeks 28-32 and 32-36 associated with overexpression, and no overexpression in gestational weeks 24-28. Preterm delivery began with premature rupture of membranes in 70.2% and spontaneous uterine activity in 29.8%. Conclusions. The Bax gene was overexpressed in preterm delivery, whereas expression of the Bcl-2 gene remained unchanged. After the 28th gestational week, apoptosis appears to be a key factor in the pathogenesis of preterm delivery.

KW - apoptosis

KW - Bax gene

KW - Bcl-2 gene

KW - placenta

KW - preterm delivery

KW - smoking in pregnancy

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